Sudan Black B Staining – Principle, Procedure, Uses
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.
Isolation of Bacillus thuringiensis (Bt) from Soil sample
Bacillus thuringiensis (Bt) is a Gram-positive, facultatively anaerobic bacterium that belongs to the B. cereus group. It is the organism that produces the proteinaceous parasporal crystals during sporulation, and these crystals are called delta-endotoxins. These are known as Cry and Cyt toxins, and it is the major insecticidal component. It is widely distributed in soil, … Read more
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
Flagella Staining – Principle, Procedure, Result
Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has been used for visualization of flagella is known as wet mount technique.
Leifson Technique for Bacterial Flagella Staining
In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from flagellated bacteria were common, as well as mutation leading to loss of motility without loss of the flagellum itself.
Thick and Thin Blood Smear Preparation
A blood smear is a simple diagnostic preparation where a drop of peripheral blood is spread on a clean slide, and it is dried for staining. It is the process used for observing blood cells and also for detecting parasites after staining methods like Giemsa. It is an important technique because it helps in demonstrating … Read more
Hanging Drop Method (Motility test) – Principle, and Procedure, Result, Uses
Hanging drop technique allows for the analysis of living microorganisms. This involves fixing the Microbial suspension into a drop of liquid over the slide of glass.
Bacterial Smear – Principle, Preparation Steps, Uses, Limitations
A bacterial smear is a thin layer of bacterial cell/culture which is placed on a clean, grease-free microscopic slide to observe a clear image under a microscope.
Kingdom Protista – Characteristics, Classification, Reproduction, Life Cycle, Examples
Kingdom Protista is described as a group of eukaryotic organisms which is neither plant nor animal nor fungus. It is the group that consists of organisms having a true nucleus and other membrane-bound organelles, and it is considered as the most diverse group among the eukaryotes. It is the historical classification used to place all … Read more
Hybridization –Types, Procedure, advantages, and limitations.
Hybridization is the process in which two organisms that belong to different varieties, subspecies, species, or even genera are crossed, and it results in the formation of a hybrid that carries genetic characters from both parents. It is the crossing that occurs naturally or by human interference where the genetic materials from two distinct parents … Read more