Lentiviral Transfection Protocol

Lentiviral transfection is a method for introducing genetic material into cells. It involves the use of lentiviruses, a type of retrovirus, to deliver the desired DNA into the target cells. Lentiviral transfection has a unique advantage over other transfection methods, in that it can infect both dividing and non-dividing cells, making it useful for a … Read more

Transfection PEI (Polyethylenimine) Protocol

Standard protein expression systems, including E. coli, frequently fail to generate folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). Utilizing a eukaryotic system, such as mammalian cells, that includes the required folding and posttranslational machinery is highly advantageous for the expression of these proteins. Using polyethylenimine, we present strategies … Read more

How is plant cell cytokinesis different from animal cell cytokinesis?

How is plant cell cytokinesis different from animal cell cytokinesis?

Cell division is a crucial process that allows organisms to grow, repair, and reproduce. One important aspect of cell division is cytokinesis, the process of separating one cell into two daughter cells. While cytokinesis is a universal process, it can vary greatly between different types of cells. In this article, we will compare and contrast … Read more

Transfection Protocols – Principle, Protocol, Uses

Transfection Protocols - Principle, Protocol, Uses

Principle of Transfection According to the definition of transfection, the external genetic material must enter the cell via the cell membrane. It is essential to highlight that the genetic material, whether DNA or RNA, is negatively charged as a result of the proteins that surround it. Therefore, in an unmanipulated environment, exogenous DNA cannot cross … Read more

Cytokinesis – Definition and Process In animal and Plant Cells

Cytokinesis - Definition and Process In animal and Plant Cells

Cytokines are small soluble protein molecules which are produced by immune cells and some other body cells. It is used as chemical messenger between cells. It help in sending signal from one cell to another cell. These molecules mainly control immunity, inflammation and formation of blood cells. Cytokines are produced by T lymphocytes, B lymphocytes, … Read more

Human Nervous System – Definition, Parts, Functions

Nervous System - Definition, Parts, Functions

Nervous system is a complex controlling and coordinating system of the body. It receives stimulus, processes the informations and gives response through muscles and glands. Human nervous system is a highly complex biological network of the human body. It is responsible for coordinating body functions, receiving sensory informations and producing different body responses. It has … Read more

Cell Cycle – Definition, Phases, Checkpoints, Regulation

Cell Cycle - Definition, Phases, Regulation

What is Cell Cycle? Cell cycle is the ordered sequence of events by which a cell grows and divides to form two genetically identical daughter cells. It is the process that ensures continuity of life by producing new cells from pre existing cells. The cell cycle is a carefully regulated process and it occurs in … Read more

Checkpoints in Cell Cycle – Types, Functions, Regulations

Checkpoints in the Cell Cycle

What is Checkpoint? A checkpoint is a control point in cell cycle. It is a internal surveillance mechanism present in eukaryotic cell. It ensures that cell division occurs in proper manner. It act like a quality control step where progression is checked before moving to next phase. It is the process where cell checks cell … Read more

Cancer Cell – Definition, Types, Morphology, Development

Cancer Cell - Definition, Types, Morphology, Development

What is Cancer Cell? Definition of Cancer Cell A cancer cell is an abnormal cell that divides uncontrollably, ignores natural growth regulation, and can invade surrounding tissues or spread to other parts of the body. Characteristics of Cancer Cells Characteristics of Cancer Cells Cancer cells are fundamentally different from normal cells in ways that make … Read more