Laboratory protocols with step-by-step methods for microbiology, molecular biology, cell biology, biochemistry, immunology, and related experiments, including reagents, procedures, conditions, and practical notes.
The Methylene Blue Reduction Test (MBRT) is an indirect microbiological quality assessment method used for testing milk. It is based on the reduction of methylene blue dye due to the metabolic activity of bacteria present in milk. The blue colour disappears when the dye is reduced. Milk containing higher bacterial activity generally takes less time
Northern blotting explained with its principle, required materials, procedure, RNA band interpretation, applications, advantages, limitations, and comparisons.
Polymerase Chain Reaction (PCR) explained with its principle, components, three cycling steps, major types, applications, advantages and limitations.
Western blotting explained: principle, materials, step-by-step procedure, protein band interpretation, controls, applications, and common limitations.
Southern blotting explained through its principle, materials, steps, DNA probe detection, band interpretation, applications, advantages, and limitations.
Plant tissue culture explained through its principle, nutrient media, major techniques, step-by-step procedure, applications, advantages and limitations.
Blood Grouping Test explained with its principle, ABO and Rh typing, materials, slide and tube methods, procedure, observations and result interpretation.
Explore protein gel staining methods, including Coomassie, silver and fluorescent stains, SDS-PAGE staining procedures, solutions, destaining and band analysis.
Micrometry explained with its principle, ocular and stage micrometers, calibration, formulas, practical procedure, calculations, and uses in microbiology.
Eastern blotting: definition, purpose, principle, procedures, result interpretation, applications, limitations, and differences from western blotting.
