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Negative Staining – Principle, Procedure, Result

Negative Staining result

In negative staining method, an acidic dye is used known as India Ink or Nigrosin. When the bacterial cells are exposed to this stain, due to the presence of acidic nature it readily gives up a hydrogen ion (proton) and the chromophore. As a result, the dye becomes negatively charged, now the bacterial cell surface deflects the stain.

Lactophenol Cotton Blue (LPCB) Mounts

Lactophenol Cotton Blue (LPCB) Mounts

The lactophenol cotton blue (LPCB) wet mount preparation is the most used method for staining and viewing fungus, and its preparation is straightforward. The formulation contains the following ingredients: As a mounting medium and staining agent, lactophenol cotton blue solution is used to prepare slides for microscopic study of fungus. Elements of fungi are dyed … Read more

Hematoxylin and Eosin (H&E) Staining – Principle, Procedure, Result, Uses

Results and Interpretation of Hematoxylin and Eosin (H&E) Staining

What is Hematoxylin and Eosin (H&E) Staining? Hematoxylin and Eosin (H&E) staining is a commonly used histological staining technique that is used to visualize the structure of cells and tissues in a sample. The staining is performed by first staining the tissue with hematoxylin, a basic dye that stains acidic structures such as the cytoplasm … Read more

Giemsa Stain – Preparation, Procedure, Principle, Composition and Application

Giemsa Stain: Staining Procedure, Principle, Result and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky stains … Read more

Acid Fast Stain – Principle, Reagents, Procedure, Result

Acid Fast Stain - Principle, Reagents, Procedure, Result

What is Acid Fast Stain? Objective of Acid Fast Stain Principle of Acid Fast Stain The principle of the Acid Fast Stain revolves around the unique characteristics of certain bacterial cells, particularly their resistance to conventional staining methods due to the presence of mycolic acid in their outer membrane. This expository explanation aims to elucidate … Read more

Chlamydia Under the Microscope

Chlamydia Under the Microscope

What is Chlamydia? Distribution of Chlamydia species Chlamydia species have a wide distribution across the world, and their prevalence varies in different regions. The family Chlamydiaceae, which includes the genus Chlamydia, is believed to have originated from the Order Chlamydiales approximately seven million years ago. However, evidence of Chlamydia trachomatis infections has been found dating … Read more

Periodic Acid-Schiff (PAS) Staining – Principle, Procedure, result, Uses

Periodic Acid-Schiff (PAS) Staining

What is Periodic Acid-Schiff (PAS) Staining? Periodic Acid-Schiff (PAS) staining is a laboratory staining technique used to detect the presence of specific sugars and carbohydrates in tissue samples. The PAS stain highlights the presence of sugars, glycoproteins, and mucopolysaccharides in biological tissues and cells. The staining process is often used in the diagnosis of diseases … Read more

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