Biology practicals covering laboratory experiments, techniques, tests, instruments, microscopy, staining, culture methods, and step-by-step procedures used in biology and microbiology practical work.
What is Chromatography? Chromatography Definition Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description Gas
Ever peered through a microscope and marveled at the microscopic universe it unveils? It’s like being Alice in her Wonderland, but instead of falling down a rabbit hole, you’re diving into the magical world of cells, bacteria, and more. Yet, have you ever wondered about the parts of this wonder-making machine and their functions? Let’s focus (pun
Glass serological pipettes are usually described as long, cylindrical measuring tools used for transferring the Liquid volumes that are needed in Cell Culture work, And many other wet-lab procedures, and it is worth mentioning that they are typically calibrated for delivering (TD) readings with graduations marked clearly,even if the spacing sometimes looks tight. These pipette’s
A centrifuge can define as a laboratory device that use rapid rotational Force to separate component’s of a mixture based on their Density difference’s, and it is considered a key Instrumentation in many biological / chemical workflows. What is Relative Centrifugal Force (RCF)? Relative Centrifugal Force (RCF) is the effective acceleration a particle experiences in
Immunoelectrophoresis is a biochemical technique which is used for the separation and identification of proteins, mainly immunoglobulins in biological samples. It is a combined method of electrophoresis and immunodiffusion. In this technique, proteins are first separated in agarose gel by applying electric current. After separation, specific antibody is added into the gel. The separated proteins
Affinity chromatography is a liquid chromatography method. It is used to separate and purify a specific biomolecule from a mixture. It is mostly used for proteins, enzymes, antibodies and nucleic acids. This method is based on the specific binding of target molecule with a ligand. The ligand is fixed on the solid stationary phase. When
Bench top centrifuge is a compact laboratory instrument which is kept on laboratory table or working bench. It is used for the separation of different components of liquid sample. It works by rotating the sample tubes at high speed with the help of a motorized rotor. During this process, a strong centrifugal force is produced.
Hematocrit centrifuge is a laboratory instrument used for separation of blood components. It works by high speed centrifugal force. The blood sample is kept in narrow capillary tube and then it is rotated very fast. During centrifugation, the heavier red blood cells (RBCs) move to the bottom of the tube. The plasma remains at the
Native PAGE is a gel electrophoresis technique used for separation of proteins and nucleic acids. It is called native because the molecule remains in its natural form. The protein is not unfolded during the process. In this method, SDS, urea and reducing agents are not used. So the protein keeps its original charge, shape and
Bioreactor is a special vessel or system used for growing living cells. It is used for microorganisms, plant cells and animal cells. It gives controlled condition for their growth and activity. In bioreactor, temperature, pH, oxygen level and nutrients are controlled properly. Due to this, the cells grow in suitable condition. The metabolic activity of
