Tollens’ Test – Principle, Procedure, Result, Application

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Oxidase Test – Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Northern Blotting – Steps, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Real-Time PCR (qPCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Plant Tissue Culture – Definition, techniques, Media composition, types, application.

Plant tissue culture

What is Plant Tissue Culture? Plant tissue culture Definition Plant tissue culture is a biotechnology technique that involves growing plant cells, tissues, or organs in a controlled, sterile environment on a nutrient medium. This method exploits the totipotency of plant cells, allowing for the rapid propagation of plants, production of disease-free specimens, and conservation of … Read more

Blotting Technique – Definition, Types, Application

Blotting Definition, Types, Application

In molecular biology and genetics, blotting is an analysis technique used for the detection of specific biomolecules (proteins, DNA or RNA) in samples of complex composition by transferring them onto a carrier such as a nitrocellulose, polyvinylidene fluoride or nylon membrane.

RNA – Definition, Structure, Types, Application

RNA - Definition, Structure, Types, Application

What is RNA? Definition of RNA RNA, or Ribonucleic acid, is a nucleic acid molecule that plays a crucial role in gene expression and protein synthesis. It is a single-stranded molecule composed of nucleotides, including adenine (A), cytosine (C), guanine (G), and uracil (U). RNA carries genetic information from DNA and acts as a template … Read more

Filtration – Definition, Mechanisms, Types, Examples, Application

Filtration - Definition, Mechanisms, Types, Examples, Application

What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to an appropriate porous material filter. Particles smaller than the filter’s pores can pass through and enter the filtrate, while larger particles are retained on the filter’s surface. Filtration is a process that is used to … Read more

Serial Dilution Method – Definition, Procedure, Application

Serial Dilutions and Plating Procedure

What is a serial dilution? Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous scientific disciplines, especially microbiology. The primary … Read more