Plant Tissue Culture – Definition, techniques, Media composition, types, application.

Plant tissue culture

What is Plant Tissue Culture? Plant tissue culture Definition Plant tissue culture is a biotechnology technique that involves growing plant cells, tissues, or organs in a controlled, sterile environment on a nutrient medium. This method exploits the totipotency of plant cells, allowing for the rapid propagation of plants, production of disease-free specimens, and conservation of … Read more

Northern Blotting – Protocol, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

RNA – Definition, Structure, Types, Application

RNA - Definition, Structure, Types, Application

What is RNA? Definition of RNA RNA, or Ribonucleic acid, is a nucleic acid molecule that plays a crucial role in gene expression and protein synthesis. It is a single-stranded molecule composed of nucleotides, including adenine (A), cytosine (C), guanine (G), and uracil (U). RNA carries genetic information from DNA and acts as a template … Read more

Giemsa Stain – Preparation, Procedure, Principle, Composition and Application

Giemsa Stain: Staining Procedure, Principle, Result and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky stains … Read more

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