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Northern Blotting – Protocol, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

RNA – Definition, Structure, Types, Application

RNA - Definition, Structure, Types, Application

What is RNA? Definition of RNA RNA, or Ribonucleic acid, is a nucleic acid molecule that plays a crucial role in gene expression and protein synthesis. It is a single-stranded molecule composed of nucleotides, including adenine (A), cytosine (C), guanine (G), and uracil (U). RNA carries genetic information from DNA and acts as a template … Read more

Giemsa Stain – Preparation, Procedure, Principle, Composition and Application

Giemsa Stain: Staining Procedure, Principle, Result and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky stains … Read more

Filtration – Definition, Mechanisms, Types, Examples, Application

Filtration - Definition, Mechanisms, Types, Examples, Application

What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to an appropriate porous material filter. Particles smaller than the filter’s pores can pass through and enter the filtrate, while larger particles are retained on the filter’s surface. Filtration is a process that is used to … Read more

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