BNO Team 
What is the Ninhydrin Test? The ninhydrin chemical test is used for determining whether an analyte contains any amines or amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The formation of a deep blue color within the test … Read more
Objective of Phenylalanine Deaminase Test Principle of Phenylalanine Deaminase Test Requirements For the test Composition of Phenylalanine agar DL-Phenylalanine: 2 gm Yeast extract: 3 gm Sodium chloride: 5 gm Disodium phosphate: 1 gm Agar: 12 gm Distilled water: 1000 ml pH: 7.3 Preparation of Phenylalanine agar slant Note: Following autoclaving, the medium should appear lighter … Read more
The Osazone test is a type of biochemical test which is used to detect reducing sugars. This test can distinguish between different types of reducing sugars by the appearance time of the complex. Osazone test can also be called Phenyl hydrozine test depending on the reagent used. Purpose Principle of Osazone Test Osazones are the … Read more
Unsaturated fish oils are especially vulnerable to oxidation, resulting in peroxides when stored in cold or freezing conditions for storage. Peroxides are precursors to breakdown products that can cause rancid taste in fat. Peroxide levels are an indicator of oxidation in the initial stages of lipid degradation. The index is less reliable in the latter … Read more
The phenol-sulfuric acid technique is a fast and easy method for determining the amount of carbohydrates present in an experiment sample. It can detect virtually all kinds of carbohydrates including di-, mono-the -, and even oligo and polysaccharides. Although the method is able to detect nearly every type of carbohydrates, their absorptivity for the various … Read more
Picric acid test is used for reducing sugars, and is a highly sensitive chemical tests. Picric acid reactions (toxic yellow crystal solid) which is also referred to chemically as 2,4 6-trinitrophenol (TNP) reacts with sugars that are reduced to create Picramic Acid that is red-colored. The Picric Acid test is made up of a sugar … Read more
Nelson-Somogyi method can be used to quantify the reduction of sugar using arsenolmolibdat and copper reagents. The principle behind the Nelson Somogyi method is the quantity of deposro oxide deposits which react with arsenomolibdate, which reduces to molybdine blue. The blue hue is determined by as absorbance. The keto and aldehyde free groups are regularly … Read more
The rapid furfural test is a type of chemical test which is used to determine the difference between fructose and glucose. The test for rapid furfural is like Molisch’s test however it utilizes concentrated hydrochloric acid instead of concentrated sulfuric acid, and the solution is then boiled. A dilute solution of sugar is added in … Read more
This is a test that is common for all carbohydrate larger than the tetroses. The test works on the basis that pentoses as well as hexoses can be dehydrated using conc. sulfuric acid, resulting in furfural or hydroxyl-methyl furfural or hydroxyl methyl furfural. These compounds condense with a-naphthol to create a purple condensation product. Molisch’s … Read more
Mucic acid test tests for lactose and galactose. The sugar is transformed into carboxylic acid using hot nitric acid. In order to produce dicarboxylic acids, aldoses are oxidized on each end of the open ring form. Chain fragmentation triggers ketoses to oxidize and produce a range of dicarboxylic acid. Galactraric acid (mucic acid) created by … Read more
