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ketone bodies

Gerhardt’s test – Principle, Procedure, Result

Avatar for Sourav Pan By Sourav Pan (M.Sc. in Microbiology) • Published on March 26, 2026 • Last Updated March 27, 2026 • 12 min read
Gerhardt's test

Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.

Categories Biochemical Test Tags Gerhardt's test, ketone bodies, principle, procedure 1 Comment

Rothera’s test – Principle, Procedure, Result

Avatar for Sourav Pan By Sourav Pan (M.Sc. in Microbiology) • Published on March 25, 2026 • 11 min read
results of Rothera’s test

Acetoacetic acid and acetone react with an alkaline solution of sodium nitroprusside to form a purple-colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected.

Categories Biochemical Test Tags ketone bodies, Rothera's test Leave a comment
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