Culture Media – Definition, Types, Composition, Use, Examples

Classification of Culture Media

When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.

Robertson’s Cooked Meat Medium (RCM Medium) – Composition, Preparation, Uses

Robertson’s Cooked Meat Medium (RCM Medium) Preparation, Composition.

Cooked Meat Medium was originally developed by Robertson to cultivate specific anaerobes isolated from wounds.
The current formulation is a modified version, sometimes referred to by the name of Chopped Meat Medium, which allows for the growth of a variety of spores and non-spore-forming strict anaerobes.

Culture Media Examples, Components and Primary Purpose

Culture Media Examples, Components and Primary Purpose

Culture Media Examples Components/Comments Primary Purpose Bile esculin agar (BEA) Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria. Differential isolation and presumptive identification of group D streptococci and enterococci Bile esculin azide agar with vancomycin Contains azide to inhibit … Read more

Columbia CNA Agar – Composition, Preparation, Uses, and Results

Columbia CNA Agar - Composition, Preparation, Uses, and Results

What is Columbia CNA Agar? Columbia CNA Agar with 5% Sheep Blood Composition of Columbia CNA Agar per Liter of Medium The composition of Columbia CNA Agar can be summarized as follows, considering the information provided: Ingredients: The base of Columbia CNA Agar is prepared using a nutritionally rich medium that includes different peptones to … Read more

Culture Media Preparation, Maintenance and Preservation

Culture Media Preparation, Maintenance and Preservation

Culture media refers to the nutrient-rich substances used in laboratories to cultivate microorganisms such as bacteria and fungi. These substances, which may be liquid or solid, give the microbes with the required nutrients, minerals, and other elements for growth and reproduction. In the late 19th century, when scientists first began to study microorganisms in depth, … Read more

Bile Esculin Agar (BEA) – Principle, Composition, Preparation, Results, Uses

Bile Esculin Agar (BEA) - Principle, Composition, Preparation, Results, Uses

What is Bile Esculin Agar (BEA)? Bile Esculin Agar (BEA) Composition Bile Esculin Agar (BEA) contains the following ingredients per liter of the medium: The final pH of the medium, when measured at 25°C, is approximately 6.6±0.2. These specific components and their concentrations are carefully selected to create a medium that is selective and differential … Read more

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