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  • Alkaline Phosphatase Test of Milk – Principle, Procedure, Result, Uses
    Alkaline Phosphatase (ALP) is an enzyme that is naturally found within all the raw milks that is utilized to determine the quality of pasteurization of milk. A complete pasteurization process can deactivate the enzyme below levels that can be detected by traditional methods. Because the stability of the heat of ALP is higher than the stability of pathogens that could be found in milk, it serves as a metric of security. However, failure for detection of ALP activity is not a mean an item is safe from pathogens.
  • Carbohydrate Fermentation Test – Sugar Fermentation Test
    The carbohydrate fermentation test can be used to determine if bacteria are able to ferment a certain carbohydrate. It is a test to determine any presence of acids or gas resulting from carbohydrate fermentation.
  • IMViC Test – Principle, Procedure, Result, Uses, Examples
    IMViC test is a group of four biochemical tests which are used in microbiology laboratory to identify and differentiate Gram-negative … Read more
  • Iodine Test – Principle, Procedure, Result, Uses
    Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.
  • Simmons Citrate Agar – Principle, Composition, Procedure, Result, uses
    Simmons Citrate Agar is a specialized solid microbiological culture medium which is used mainly to differentiate Gram-negative bacteria especially the … Read more
  • Gelatin Hydrolysis Test – Purpose, Principle, Procedure, Result.
    The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.
  • CAMP Test – Principle, Procedure, Result, Uses
    CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.
  • Reverse CAMP test – Principle, Procedure, Result, Uses
    In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.
  • Biuret Test For Protein – Principle, Procedure, Result, Uses
    Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.
  • Tollens’ Test – Principle, Procedure, Result, Application
    It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

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