Microbiology Notes Topcis

Uncover the Microscopic Marvels – Dive into the World of Tiny Titans!

Zoology Notes Topics

Explore the Animal Kingdom – From Amoebas to Zebras!

Biology Practicals

Master the Art of Science – Hands-On Learning Starts Here!

Microorganisms

Meet the Invisible Invincibles – Discover Life Beyond the Naked Eye!

Other Topics

Latest Notes

  • Gel Staining Procedure for PAGE
    Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.
  • Laboratory Rules – Basic Rules Of A Microbiology Laboratory
    A well-established microbiology laboratory is necessary for the work of microorganisms. The students/researchers/scientists must follow the basic rules while working with microorganisms.
  • Measurement Of Microorganisms – Micrometry
    A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.
  • Giolitti-Cantoni Broth for Staphylococcus aureus
    Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.
  • Northern Blotting – Protocol, Principle, Application, Result
    Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.
  • Western Blot – Protocol, Principle, Result.
    In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
  • Southern Blotting Definition, Principle, Steps, Importance
    Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
  • Eastern blot – Protocol, Principle, Application
    A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.
  • Methylene Blue Reduction Test
    Methylene Blue Reduction Test also known as mbrt test. It … Read more
  • Plant Tissue Culture Definition, techniques, Media composition, types, application.
    Before learning about plant tissue culture, first you have to … Read more
  • Dot Blot – Protocol, Principle, Definition.
    Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
  • Restriction Digest – Protocol, Principle, result
    The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.