Pipetting – Technique, Definition, Types

Pipetting - Technique, Definition, Types

Pipetting Definition Pipetting is the process of using a pipettor to measure and dispense small volumes of liquid. It is a fundamental technique used in many laboratory procedures, including preparing solutions, dispensing samples for analysis, and performing various types of assays. When pipetting, the user first sets the desired volume on the pipettor by adjusting … Read more

Positive Displacement Pipette – Principle, Procedure, Applications, Advantages

Positive Displacement Pipette - Principle, Procedure, Applications, Advantages

Positive displacement pipettes are characterised by piston-driven displacement. The piston in a positive displacement pipette makes direct contact with the sample, which allows the aspiration force to remain constant. Air displacement pipettes accurately dispense the majority of liquids. However, the precision may be affected negatively by the solution’s viscosity, volatility, surface tension, and temperature. For … Read more

Air Displacement Pipetting – Principle, Procedure, Applications

Air Displacement Pipetting - Principle, Procedure, Applications

An air displacement pipette (also known as a “volumetric pipette” or “mechanical pipette”) is a type of liquid handling tool commonly used in laboratory settings. It uses air displacement to draw a specific volume of liquid into the pipette’s barrel. The volume of liquid is determined by the calibrated volume markings on the barrel of … Read more

How to Use a Micropipette?

How to Use a Micropipette?

Standard laboratory equipment for measuring and transferring minute amounts of liquid includes micropipettors. You’ll put them to use all through the semester and into subsequent upper-level courses. If you want reliable results from your tests, you must become an expert user of these tools. Objective Parts of a micropipette Different sizes of micropipettes The laboratory’s … Read more

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa Principle The MacConkey Agar is a specialized culture medium widely used in microbiology for the isolation, identification, and differentiation of gram-negative bacteria, particularly those belonging to the Enterobacteriaceae family. The principle of MacConkey Agar lies in its combination of selective and differential properties, making it an effective tool in microbiological analyses. The medium’s … Read more

Microinjection – Definition, Types, Principle, Steps Applications

Microinjection - Definition, Types, Principle, Steps Applications

What is Microinjection? Definition of Microinjection Microinjection is a precise technique used to introduce DNA or other genetic materials directly into a cell using a micropipette or fine glass needle. It is widely employed in genetic engineering, genome editing, and various biomedical research areas. Principle of Microinjection Microinjection operates on the fundamental principle of directly … Read more

Filtration – Definition, Mechanisms, Types, Examples, Application

Filtration - Definition, Mechanisms, Types, Examples, Application

What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to an appropriate porous material filter. Particles smaller than the filter’s pores can pass through and enter the filtrate, while larger particles are retained on the filter’s surface. Filtration is a process that is used to … Read more

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