What is Chlamydia? Distribution of Chlamydia species Chlamydia species have a wide distribution across the world, and their prevalence varies in different regions. The family Chlamydiaceae, which includes the genus Chlamydia, is believed to have originated from the Order Chlamydiales approximately seven million years ago. However, evidence of Chlamydia trachomatis infections has been found dating … Read more
What is Acid Fast Stain? Objective of Acid Fast Stain Principle of Acid Fast Stain The principle of the Acid Fast Stain revolves around the unique characteristics of certain bacterial cells, particularly their resistance to conventional staining methods due to the presence of mycolic acid in their outer membrane. This expository explanation aims to elucidate … Read more
Gram-staining used to differentiate between Gram-Positive and Gram-Negative Bacteria.
The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky stains … Read more
What is Hematoxylin and Eosin (H&E) Staining? Hematoxylin and Eosin (H&E) staining is a commonly used histological staining technique that is used to visualize the structure of cells and tissues in a sample. The staining is performed by first staining the tissue with hematoxylin, a basic dye that stains acidic structures such as the cytoplasm … Read more
The lactophenol cotton blue (LPCB) wet mount preparation is the most used method for staining and viewing fungus, and its preparation is straightforward. The formulation contains the following ingredients: As a mounting medium and staining agent, lactophenol cotton blue solution is used to prepare slides for microscopic study of fungus. Elements of fungi are dyed … Read more
Trichrome stains, such as Masson’s Trichrome, derive its name from the three dyes utilised in the staining process. These three dyes are used to selectively stain muscle, collagen fibres, fibrin, and erythrocytes using acid-base chemistry. Initial placement of tissue slices in Bouin’s solution. As a mordant, Bouin’s solution links the dye to the specific tissue … Read more
Since the late 1950s and early 1960s, the positive staining approach has been employed to increase the contrast of biological materials (tissues and cell structures, viruses, etc.). The samples are treated in heavy metal salt solutions that react with cellular structures using this technique and negative staining. Uranyl acetate and lead citrate are the most … Read more
What is Wheatley Trichrome Staining? In parasitology, intestinal protozoans are typically detected and identified from faeces samples using a specialised permanent technique called Wheatley Trichrome staining. Wheatley Trichrome Stain is suggested for use in qualitative methods for the detection, identification, and distinction of intestinal protozoa from background material. Typically, PVA-fixed or Schaudinn’s solution-preserved faecal specimens … Read more
Romanowsky staining, also known as Romanowsky–Giemsa staining, is a classic staining technique that paved the way for several different but similar stains widely used in haematology (the study of blood) and cytopathology (the study of cells) (the study of diseased cells). Romanowsky stains are used to distinguish cells for microscopic study in pathological materials, particularly … Read more
