DNA Library – Types, Construction, Applications

DNA Library - Types, Construction, Applications

What is DNA Library (Genomic, cDNA)? Types of DNA Library There are two main types of DNA libraries: genomic libraries, and cDNA libraries. 1. Genomic Library 2. cDNA Library Genomic Library Construction Steps Genomic Library Construction Steps Creating a cDNA library begins with mRNA, which carries genetic instructions from DNA to ribosomes for protein synthesis. … Read more

Emulsion PCR – Principle, Procedure, Advantages, Limitations, Uses

Emulsion PCR - Principle, Procedure, Advantages, Limitations, Uses

What is Emulsion PCR? Principle of Emulsion PCR Emulsion PCR (polymerase chain reaction) is a sophisticated technique designed to amplify individual DNA templates in a high-throughput manner by partitioning the DNA into tiny water-in-oil droplets. Each droplet serves as an independent microreactor, allowing for the separate amplification of distinct DNA fragments. This method leverages the … Read more

TaqMan Probe – Definition, Principle, Applications

TaqMan - Definition, Principle, Applications

What is TaqMan Probe? Definition of TaqMan TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA. Principle of TaqMan TaqMan technology … Read more

Differences Between Sensitivity, Specificity, False positive, False negative

Differences Between Sensitivity, Specificity, False positive, False negative

In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

What is Protein Synthesis? Protein synthesis is a fundamental biological process that occurs within cells, responsible for the formation of proteins through the assembly of amino acid chains, termed polypeptides. This process is pivotal to the central dogma of genetics and is instrumental in maintaining cellular function and structure. The process of protein synthesis can … Read more

Hybridization Probes – definition, uses, Preparation, Types.

The firefly (ATP-dependent) lucifer-luciferinase reaction

Hybridization Probes Definition Hybridization probes are a small nucleotide sequence that is used for the detection of complementary sequences (DNA or RNA) in a nucleic acid sample. DNA Probes detects the complementary DNA with the help of a hybridization reaction. This is achieved by permitting the probes to base pair with the sample nucleic acid … Read more

cDNA Cloning – Definition, Principle, Steps, Applications

Complementary DNA or cDNA cloning - Principle, Steps 

What is cDNA cloning? Definition of Complementary DNA or cDNA Complementary DNA (cDNA) is a form of DNA synthesized from a messenger RNA (mRNA) template through the action of the enzyme reverse transcriptase. It represents the coding sequence of genes and is commonly used in gene cloning and expression studies. cDNA Cloning Definition cDNA cloning … Read more

Translocation in Prokaryotes – Components, Steps, Enzymes, Sites

Prokaryotic Translation Steps, Requirements.

In molecular biology and genetics, translation is the process in which ribosomes in the cytoplasm or endoplasmic reticulum synthesize proteins after the process of transcription of DNA to RNA in the cell’s nucleus. The entire process is called gene expression.

Central Dogma Theory of Molecular Biology and Genetic Code

Central Dogma Replication, Transcription, Translation

DNA is the full genetic information that determines the nature and function that an animal has. Proteins are made by the genetic code contained in DNA. Conversion of DNA encoded data to RNA is necessary to make proteins. So, in the majority of cells, genetic information is transferred from – DNA to RNA, and then to protein. The transfer of information is controlled by three different processes that help in the transfer of genetic information as well as its transformation into a different form:

Differences Between Sensitivity, Specificity, False positive, False negative

Differences Between Sensitivity, Specificity, False positive, False negative

In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more

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