Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

TaqMan Probe – Definition, Principle, Applications

TaqMan - Definition, Principle, Applications

What is TaqMan Probe? Definition of TaqMan TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA. Principle of TaqMan TaqMan technology … Read more

Differences Between Sensitivity, Specificity, False positive, False negative

Differences Between Sensitivity, Specificity, False positive, False negative

In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

What is Protein Synthesis? Protein synthesis is a fundamental biological process that occurs within cells, responsible for the formation of proteins through the assembly of amino acid chains, termed polypeptides. This process is pivotal to the central dogma of genetics and is instrumental in maintaining cellular function and structure. The process of protein synthesis can … Read more

Hybridization Probes – definition, uses, Preparation, Types.

The firefly (ATP-dependent) lucifer-luciferinase reaction

Hybridization Probes Definition Hybridization probes are a small nucleotide sequence that is used for the detection of complementary sequences (DNA or RNA) in a nucleic acid sample. DNA Probes detects the complementary DNA with the help of a hybridization reaction. This is achieved by permitting the probes to base pair with the sample nucleic acid … Read more

cDNA Cloning – Definition, Principle, Steps, Applications

Complementary DNA or cDNA cloning - Principle, Steps 

What is cDNA cloning? Definition of Complementary DNA or cDNA Complementary DNA (cDNA) is a form of DNA synthesized from a messenger RNA (mRNA) template through the action of the enzyme reverse transcriptase. It represents the coding sequence of genes and is commonly used in gene cloning and expression studies. cDNA Cloning Definition cDNA cloning … Read more

Central Dogma Theory of Molecular Biology and Genetic Code

Central Dogma Replication, Transcription, Translation

DNA is the full genetic information that determines the nature and function that an animal has. Proteins are made by the genetic code contained in DNA. Conversion of DNA encoded data to RNA is necessary to make proteins. So, in the majority of cells, genetic information is transferred from – DNA to RNA, and then to protein. The transfer of information is controlled by three different processes that help in the transfer of genetic information as well as its transformation into a different form:

Differences Between Sensitivity, Specificity, False positive, False negative

Differences Between Sensitivity, Specificity, False positive, False negative

In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more

Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Microprojectile Bombardment – Definition, Components, Steps, Applications

Microprojectile Bombardment - Definition, Components, Steps, Applications

What is Microprojectile Bombardment? Definition of Microprojectile Bombardment Microprojectile bombardment, also known as biolistics or gene gun delivery, is a method used to introduce foreign genetic material into cells or tissues by accelerating microscopic particles coated with the desired DNA, RNA, or proteins at high velocities, facilitating direct gene transfer. This technique is commonly employed … Read more

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×