Molecular biology notes covering DNA, RNA, genes, replication, transcription, translation, gene regulation, recombinant DNA, molecular techniques, genome organization, and protein expression.
What is replicative transposition? Replicative transposition is a type of transposition in which the transposable element is duplicated during its movement from one site to another. It is the process where the original transposable element remains at the donor site and a new copy is inserted into the target DNA. This is referred to as … Read more
Transposable elements can be described as mobile genetic segments that is able to move from one position of the genome to another without the need of any homology. It is the process where small DNA sequences is shifted or sometimes replicated inside the chromosomes, and this movement is referred to as transposition. It is seen … Read more
Okazaki fragment is the short segment of DNA that is synthesized on the lagging strand during DNA replication. It is formed because the DNA polymerase can extend the new strand only in the 5’–3’ direction, so the strand that is oriented in the 3’–5’ direction is produced in a discontinuous manner. It is initiated by … Read more
Post-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an primary RNA transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell.
DNA replication is the process by which genetic material is copied before cell division happens, it’s considered essential for inheritance. Among different models, the main ones observed are Theta model, Rolling Circle model, and Linear DNA replication. Each model are used by different organisms or DNA types (like plasmids, viruses, or eukaryotic chromosomes). The three … Read more
A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.
What is Electroporation? Electroporation is a fascinating technique that temporarily makes cell membranes more permeable by applying brief electric pulses. This method, widely used in labs and medical settings, allows substances like DNA, drugs, or proteins to enter cells by creating tiny pores in the membrane. Imagine zapping cells with a controlled jolt of electricity—this … Read more
What is Semiconductor Sequencing? Semiconductor sequencing is the integration of microchip technology with DNA sequencing where electrical signals replace the detection of light. This sequencing method is unique because it uses complementary metal-oxide semiconductor chips associated with sequencing by synthesis chemistry; so, in this respect, it opens up opportunities to make DNA analysis quicker and … Read more
What is Theta model of DNA Replication? The theta model of DNA replication is a mechanism for DNA replication which occurs in circular DNA molecules, in particular, those of bacteria, and also the mitochondria and chloroplasts. True to its name, this replication process creates an intermediate structure during replication that resembles the Greek letter theta … Read more
What is Telomerase? Telomerase (terminal transferase) is a ribonucleoprotein enzyme that extends telomeres, the repetitive nucleotide sequences at the ends of eukaryotic chromosomes. Telomeres function as protective caps, stopping chromosome ends from being erroneously perceived as DNA damage or from bonding with neighboring chromosomes. Most eukaryotes use telomerase to maintain their telomeres, but some organisms, … Read more
