What are Gram-negative bacteria? Cell wall of the Gram-negative is more complicated than the Gram-positive cell wall. The amount of peptidoglycan present in the Gram-negative cell wall is considerably lower than that of that of the cell’s Gram positive wall. There are only a few layers of peptidoglycan (2-8 millimeters) are visible in the cell … Read more
Bacterial Cell Wall Characteristics of Peptidoglycan Peptidoglycan is a fundamental component of bacterial cell walls, forming a complex, mesh-like structure known as the sacculus. This structure is crucial for maintaining cell shape and integrity. Here are the key characteristics of peptidoglycan: Cell membrane of Gram-Positive Cell Wall The cell membrane of Gram-positive bacteria is a … Read more
Archaeal Cell Wall Introduction One Membrane Archaea Double Membranes of Archaea What is S-Layer? The S-layer, or surface layer, is a fundamental component of the cell envelope in many archaea and some bacteria. It represents one of the most common cell wall variations, particularly among Crenarchaea. This layer consists primarily of proteins that self-assemble into … Read more
What is Cell Wall? Definition of Cell Wall A cell wall is a rigid, protective layer that surrounds the cell membrane in plants, fungi, algae, and some prokaryotes, providing structural support, shape, and protection. Bacterial Cell Wall Types Based on the result of gram staining it is stated that there are two types of cell … Read more
In the early 20th century there was no treatment to treat infections caused by bacteria. This included tuberculosis and pneumonia, as well as the rheumatic and gonorrhea diseases, and infections of the urinary tract. However, in 1929 the the bacteriologist Alexander Fleming discovered the first real antibiotic, penicillin, heralding a new era of medical science. … Read more
What are Cell wall synthesis inhibitors? Cell wall synthesis inhibitors encompass antibiotics like β-lactams and glycopeptides. These antibiotics play a crucial role in treating infections by targeting the bacterial cell wall, an essential structure for bacterial survival. Understanding the mechanisms and types of these inhibitors provides insight into their therapeutic applications. β-Lactam Antibiotics β-lactam antibiotics … Read more
What is Karyotyping? Karyotyping: What’s the Deal? Definition of Karyotyping Karyotyping is a diagnostic technique that examines an individual’s chromosomes to identify genetic abnormalities, structural changes, and chromosome number variations. What is a karyotype? Karyotyping Procedure/Steps of Karyotyping Staining and banding Staining and Banding techniques used in karyotyping: Applications of Karyotyping Medical Genetics: Prenatal Diagnostics: … Read more
What is DNA Library (Genomic, cDNA)? Types of DNA Library There are two main types of DNA libraries: genomic libraries, and cDNA libraries. 1. Genomic Library 2. cDNA Library Genomic Library Construction Steps Genomic Library Construction Steps Creating a cDNA library begins with mRNA, which carries genetic instructions from DNA to ribosomes for protein synthesis. … Read more
What is The Wobble Hypothesis? The Wobble Hypothesis, proposed by Francis Crick in 1966, provides an explanation for the degeneracy of the genetic code. Degeneracy refers to the fact that multiple codons can code for the same amino acid. According to the Wobble Hypothesis, the precise pairing between the bases of the codon and the … Read more
What is Emulsion PCR? Principle of Emulsion PCR Emulsion PCR (polymerase chain reaction) is a sophisticated technique designed to amplify individual DNA templates in a high-throughput manner by partitioning the DNA into tiny water-in-oil droplets. Each droplet serves as an independent microreactor, allowing for the separate amplification of distinct DNA fragments. This method leverages the … Read more
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