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History of Microbiology 

History of Microbiology 

The Emergence of Microbiology: Uncovering Microbes Microbiology, the study of microscopic living organisms, is a relatively recent scientific field that has significantly advanced our understanding of biology and disease. The foundation of microbiology can be traced back to the mid-19th century, during a period of considerable growth and development in the biological sciences. Key Developments … Read more

Bacterial Growth Curve Protocol

Bacterial Growth Curve Protocol

What is Bacterial Growth Curve? The bacterial growth curve is a graphical representation of the growth of a population of bacteria in a closed system over time. It typically consists of four phases: The shape and duration of each phase can vary depending on the bacterial species, the nutrients available, and other environmental factors. Understanding … Read more

TaqMan Probe – Definition, Principle, Applications

TaqMan - Definition, Principle, Applications

What is TaqMan Probe? Definition of TaqMan TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA. Principle of TaqMan TaqMan technology … Read more

56 Pioneers in Microbiology and their Great Contribution

56 Pioneers in Microbiology and their Great Contribution

Here is the list of Top 56 Pioneers in Microbiology and their Great Contribution Antonie van Leeuwenhoek (1632–1723): The First Microbiologist Antonie van Leeuwenhoek was a Dutch scientist who is often considered the father of microbiology. He was the first to observe bacteria and other microorganisms using a microscope that he designed and built himself. … Read more

Zygote – Definition, Formation, Development, Example

Zygote - Definition, Formation, Development, Example

What is a Zygote? A zygote is a crucial concept in biology, representing the very first stage of a new organism’s development following the fertilization event. The term “zygote” (pronounced /ˈzaɪˌɡoʊt/) originates from the Ancient Greek word ζυγωτός (zygōtós), meaning ‘joined’ or ‘yoked,’ derived from ζυγοῦν (zygoun), which means ‘to join’ or ‘to yoke’. This … Read more

History, Branches, and Career Opportunities of Microbiology

Glorious History of Microbiology

What is Microbiology? Microbiology, derived from the Greek words μῑκρός (mīkros) meaning ‘small’, βίος (bíos) meaning ‘life’, and -λογία (-logía) meaning ‘study of’, is the scientific discipline focused on the study of microorganisms. These microorganisms can be unicellular (single-celled), multicellular (comprising multiple cells), or acellular (lacking cellular structure). The field encompasses several sub-disciplines, including virology, … Read more

Differences Between Sensitivity, Specificity, False positive, False negative

Differences Between Sensitivity, Specificity, False positive, False negative

In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

Protein Synthesis (Translation)- Definition, Steps, Sites, Machinery

What is Protein Synthesis? Protein synthesis is a fundamental biological process that occurs within cells, responsible for the formation of proteins through the assembly of amino acid chains, termed polypeptides. This process is pivotal to the central dogma of genetics and is instrumental in maintaining cellular function and structure. The process of protein synthesis can … Read more

Hybridization Probes – definition, uses, Preparation, Types.

The firefly (ATP-dependent) lucifer-luciferinase reaction

Hybridization Probes Definition Hybridization probes are a small nucleotide sequence that is used for the detection of complementary sequences (DNA or RNA) in a nucleic acid sample. DNA Probes detects the complementary DNA with the help of a hybridization reaction. This is achieved by permitting the probes to base pair with the sample nucleic acid … Read more

cDNA Cloning – Definition, Principle, Steps, Applications

Complementary DNA or cDNA cloning - Principle, Steps 

What is cDNA cloning? Definition of Complementary DNA or cDNA Complementary DNA (cDNA) is a form of DNA synthesized from a messenger RNA (mRNA) template through the action of the enzyme reverse transcriptase. It represents the coding sequence of genes and is commonly used in gene cloning and expression studies. cDNA Cloning Definition cDNA cloning … Read more

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