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Oil Immersion – Technique, objectives, Resolving Power, Used for, Types.

Oil Immersion technique, objectives, Resolving Power, Used for, Types.

What is Oil Immersion? What is Microscopic Resolution? Microscopic Resolution Definition The microscopic resolution also is known as resolving power. Resolution is defined as the ability of a lens to separate or distinguish between small objects that are close together or the ability to distinguish two adjacent points as distinct and separate. Equation of Microscopic … Read more

Dark Field Microscopy – Principle, Parts, Procedure, Uses

Dark Field Microscopy - Principle, Parts, Procedure, Uses

What is Dark Field Microscopy? Definition of Dark Field Microscope A darkfield microscope is a type of optical microscope that uses oblique illumination to light the specimen, causing it to appear bright against a dark background. This technique enhances the visibility of transparent and living specimens by excluding the unscattered light from the image. Principle … Read more

Fluorescence Microscopy – Definition, Principle, Parts, Uses, Examples

Fluorescence Microscopy - Definition, Principle, Parts, Uses, Examples

The concept of using fluorescence to study biological materials dates back to the late 19th century, but the first practical fluorescence microscope was not developed until the 1930s. In 1938, Robert Goldstein and Richard Manly, researchers at the University of California, Berkeley, published a paper describing the use of fluorescence to study cells and tissues … Read more

Diaphragm of a Microscope – Definition, Types, Mechanism, Functions

Diaphragm of a Microscope - Definition, Types, Mechanism, Functions

What is Diaphragm of a Microscope? History of Diaphragm Definition of Diaphragm of a Microscope The diaphragm of a microscope is a component that controls the amount of light that passes through the specimen being observed. It is typically located between the light source and the stage, and consists of a series of adjustable blades … Read more

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy - Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy, short for Ultraviolet-Visible Spectroscopy, is an analytical technique widely used in the fields of chemistry, physics, biochemistry, and molecular biology. It’s based on the absorption or transmission of UV and visible light by substances. At its core, UV-Vis Spectroscopy involves shining ultraviolet or visible light through a sample and measuring how much of … Read more

Objective Lenses – Principle, Types, Specification, Uses

Objective Lenses - Principle, Types, Specification, Uses

What is Objective Lens? Purpose of Objective Lenses Objective lenses serve several key purposes in optical instruments, playing a critical role in magnification, image formation, and resolution. The primary purposes of objective lenses include: Specification of Objective Lens The specification of an objective lens encompasses several key parameters that define its performance and suitability for … Read more

Microscope Condenser – Types, Parts, Mechanism, Uses

Microscope Condenser - Types, Parts, Mechanism, Uses

A microscope condenser is an integral optical component in the realm of microscopy, designed to focus and direct light onto the specimen being examined. This specialized lens system is strategically positioned beneath the microscope stage, a placement crucial for optimal illumination. The primary function of the condenser is to gather light from the microscope’s light … Read more

Parts of a Microscope and Their Functions

Parts of a Microscope and Their Functions

What are Microscopes? Parts of a Microscope The microscope parts are divided into two main categories,  such as; Structural parts of a microscope and their functions Structural parts of a microscope Location Function Head (Body Tube) Situated at the microscope’s apex. Serves as a pivotal connector, linking the eyepiece to the objective lenses, ensuring a … Read more

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

What is Ion Exchange Chromatography? Definition of Ion Exchange Chromatography Ion exchange chromatography is a separation technique that separates ions and polar molecules based on their affinity to ion exchangers. It involves the reversible exchange of ions between the target ions in the sample solution and ions attached to an ion exchanger. Working Principle of … Read more

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