UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy - Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis spectroscopy (Ultraviolet-visible spectroscopy) is an analytical technique used to measure the amount of ultraviolet and visible light absorbed or transmitted by a chemical sample. It measures light in UV and visible region. The reading is taken as absorbance or transmittance. This technique relies on interaction between electromagnetic radiation and matter. When molecules are exposed … Read more

UV Spectroscopy – Principle, Parts, Protocol, Applications

UV Spectroscopy - Principle, Instruments, Applications

UV-Visible (UV-Vis) spectroscopy is an analytical technique used to measure the absorption or transmission of ultraviolet and visible light by a sample. It works in UV region (about 190–400 nm) and visible region (about 400–800 nm). It is a fast and simple method used in both qualitative and quantitative analysis. The absorption is recorded as … Read more

Objective Lenses – Principle, Types, Specification, Uses

Objective Lenses - Principle, Types, Specification, Uses

Objective lens is the most important lens of an optical microscope, which is kept closest to the specimen. It is the first lens that collects the light coming from the specimen (emitted or reflected). It bends the light rays and forms a real, magnified image at an intermediate image plane. This primary image is then … Read more

Ion Exchange Chromatography – Principle, Protocol, Applications, Examples

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

Ion Exchange Chromatography (IEC) is a chromatographic separation technique used to separate and purify molecules based on their net surface charge. It works mainly for charged biomolecules like proteins, peptides, amino acids and nucleic acids. In this method, separation depends on electrostatic attraction between the sample and the charged stationary phase. Ion exchange chromatography is … Read more

Disposable Glass Pasteur Pipettes

Disposable Glass Pasteur Pipettes

Ever wondered about the little scientific marvels you hold in your hand during chemistry or biology labs? We’re shining a spotlight on one such unsung hero of the scientific world – disposable glass pasteur pipettes.  These handy tools, so often taken for granted, have a fascinating history and a crucial role in your experimental success. Let’s dive in and … Read more

BOD Incubator – Principle, Parts, Procedure, Application

BOD Incubator - Definition, Principle, Components, Procedure, Application

A BOD incubator is a laboratory instrument that is used for maintaining a controlled temperature environment required for the estimation of Biochemical Oxygen Demand of water samples. It is designed to operate both at heating and cooling conditions which is different from ordinary incubators. It is mainly adjusted at a fixed temperature of 20°C which … Read more