PH Meter – Principle, Parts, Types, Procedure, Calibration, Application

PH Meter - Principle, Parts, Types, Procedure, Calibration, Application, Examples

pH meter is an instrument used to measure pH of a solution. It measures the hydrogen ion activity in water based solution. It is used to know whether solution is acidic or alkaline. It works on potentiometry principle. It measures the difference in electrical potential between a measuring electrode and a reference electrode. The measuring

HPLC – Principle, Instrumentation, Types, Uses, Diagram

High-Performance Liquid Chromatography (HPLC)

HPLC (High-performance liquid chromatography) is a liquid chromatography technique used to separate, identify and quantify different components present in a liquid mixture. It is also called high-pressure liquid chromatography. It is used when accurate separation is needed in a mixture. In this technique, a liquid solvent is used as mobile phase and the column is

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy - Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis spectroscopy (Ultraviolet-visible spectroscopy) is an analytical technique used to measure the amount of ultraviolet and visible light absorbed or transmitted by a chemical sample. It measures light in UV and visible region. The reading is taken as absorbance or transmittance. This technique relies on interaction between electromagnetic radiation and matter. When molecules are exposed

Ion Exchange Chromatography – Principle, Protocol, Applications, Examples

Ion Exchange Chromatography – Definition, Principle, Protocol, Applications, Examples

Ion Exchange Chromatography (IEC) is a chromatographic separation technique used to separate and purify molecules based on their net surface charge. It works mainly for charged biomolecules like proteins, peptides, amino acids and nucleic acids. In this method, separation depends on electrostatic attraction between the sample and the charged stationary phase. Ion exchange chromatography is

Disposable Glass Pasteur Pipettes

Disposable Glass Pasteur Pipettes

Ever wondered about the little scientific marvels you hold in your hand during chemistry or biology labs? We’re shining a spotlight on one such unsung hero of the scientific world – disposable glass pasteur pipettes.  These handy tools, so often taken for granted, have a fascinating history and a crucial role in your experimental success. Let’s dive in and

BOD Incubator – Principle, Parts, Procedure, Application

BOD Incubator - Definition, Principle, Components, Procedure, Application

A BOD incubator is a laboratory instrument that is used for maintaining a controlled temperature environment required for the estimation of Biochemical Oxygen Demand of water samples. It is designed to operate both at heating and cooling conditions which is different from ordinary incubators. It is mainly adjusted at a fixed temperature of 20°C which