Ion Exchange Chromatography – Principle, Protocol, Applications, Examples
Ion Exchange Chromatography (IEC) is a chromatographic separation technique used to separate and purify molecules based on their net surface charge. It works mainly for charged biomolecules like proteins, peptides, amino acids and nucleic acids. In this method, separation depends on electrostatic attraction between the sample and the charged stationary phase. Ion exchange chromatography is … Read more