Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

Real-Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Microprojectile Bombardment – Definition, Components, Steps, Applications

Microprojectile Bombardment - Definition, Components, Steps, Applications

What is Microprojectile Bombardment? Definition of Microprojectile Bombardment Microprojectile bombardment, also known as biolistics or gene gun delivery, is a method used to introduce foreign genetic material into cells or tissues by accelerating microscopic particles coated with the desired DNA, RNA, or proteins at high velocities, facilitating direct gene transfer. This technique is commonly employed … Read more

What is DNA Replication? – An In-Depth Guide to DNA Replication for Students

DNA Replication Mechanism, Definition, Requirements, Steps

What is DNA Replication? In the realm of molecular biology, DNA replication stands as a pivotal biological mechanism responsible for generating two congruent DNA replicas from a singular original DNA molecule. This process is ubiquitous across all living entities, serving as the cornerstone for biological inheritance. DNA replication is not merely a routine cellular activity; … Read more

What is Gene Expression? – Definition, Stages, Importance

What is Gene Expression? - Definition, Stages, Importance

What is gene expression? Gene expression definition Gene expression is the process by which the genetic information encoded in a gene is used to produce a functional product, typically a protein or a specific RNA molecule. Stages in Gene Expression 1. Transcription 2. Translation What is gene expression profiling? Methods or Steps of gene expression … Read more

Post-transcriptional Modification – Definition, Types, Process, Importance

Post-transcriptional modification

Post-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an primary RNA transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell.

Restriction Enzyme (Restriction Endonuclease) – Definition, Types, Examples, Functions

Restriction Enzyme (Restriction Endonuclease) - Definition, Types, Examples, Functions

What is Restriction Enzyme (Restriction Endonuclease)? Definition of Restriction Enzyme (Restriction Endonuclease) A restriction enzyme, also known as a restriction endonuclease, is an enzyme that cuts DNA at specific recognition sites, called restriction sites. It is primarily found in bacteria and archaea and serves as a defense mechanism against invading viruses. Restriction enzymes are commonly … Read more

Types of RNA with Structure and Functions

Types of RNA with Structure and Functions

What is RNA? Different Types of RNA and Their Functions RNA, or Ribonucleic acid, is a crucial molecule synthesized by RNA polymerase from DNA. It serves various functions in the cell, including protein-coding and non-coding roles. Understanding the different types of RNA is essential for comprehending their specific functions. Let’s delve into the main types … Read more

Pulse Field Gel Electrophoresis (PFGE) Protocol

Pulse Field Gel Electrophoresis (PFGE) Protocol

Pulsed Field Gel Electrophoresis (PFGE) is a highly effective genotyping technique used for the separation and analysis of large DNA molecules, such as entire genomic DNA. It involves digesting the DNA with specific restriction enzymes and applying it to a gel matrix under an electric field that periodically changes direction. PFGE is a modified version … Read more

Prokaryotic DNA Replication – Initiation, Elongation, Termination

Prokaryotic DNA Replication - Initiation, Elongation, Termination

What is Prokaryotic DNA Replication? Structure of Ori C Mechanism of prokaryotic dna replication The synthesis of a DNA molecule can be divided into three stages:  distinguished both by the reactions taking place and by the enzymes required. As you will find here and in the next two chapters, synthesis of the major information containing … Read more

DNA Sequencing – Definition, Principle, Steps, Types, Applications

DNA Sequencing - Definition, Principle, Steps, Types, Applications

What is DNA Sequencing? Definition of DNA Sequencing DNA sequencing refers to the techniques used to determine the order of the nucleotide bases adenine, guanine, cytosine, and thymine within a DNA molecule. Methods of DNA Sequencing DNA sequencing is a crucial technique used in various fields of biological research, allowing scientists to unravel the genetic … Read more

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×