Questions and Answers

What is unique about transduction compared to normal bacteriophage infection?
A) Transduction transfers DNA from the chromosome of one cell to another.
B) The bacteriophage does not erupt from an infected cell during transduction.
C) The bacteriophage takes fragments of the cell with it during transduction.

Donor DNA is combined with recipient DNA by homologous recombination.
A) True
B) False

In transformation, both strands of donor DNA enter the recipient cell.
A) True
B) False

When DNA from an ampicillin-resistant organism is brought into an ampicillin-sensitive organism by transformation and the product is grown on agar containing ampicillin:
A) Only the transformed cells will grow
B) Only donor cells will grow
C) All cells grow
D) No cells grow
E) Only ampicillin-sensitive cells grow

The mismatch repair system will repair:
A) Only mismatching donor sequences
B) Only mismatching recipient sequences
C) Both sides of mismatching sequences
D) Either the donor or the recipient strand

DNA transformation involves the transfer of DNA via:
A) Pili
B) Naked DNA in solution
C) A restriction endonuclease
D) A bacteriophage

Plasmids are small pieces of DNA that may carry genetic information for antibiotic resistance.
True
False

The pilus is found on both the donor and recipient cells.
A) True
B) False

If one donor cell is mixed with 20 recipient cells, eventually _ cells will become donors.
2
5
10
15
20

During bacterial conjugation, plasmid replication occurs in:
A) The donor only.
B) The recipient only.
C) Both the donor and recipient.
D) Either the donor or the recipient, but not both.
E) Neither the donor nor the recipient.