Immunofluorescence Quenching Solution Application
Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.
Components required
The following components are required to prepare 1L of Immunofluorescence Quenching Solution.
| Component | Amount | Concentration |
| Glycine (mw: 75.07 g/mol) | 3.75 g | 0.05 M |
| NH4Cl (mw: 53.49 g/mol) | 2.67 g | 0.05 M |
Immunofluorescence Quenching Solution Preparation
- Prepare 800 mL of distilled water in a suitable container.
- Add 3.75 g of Glycine to the solution.
- Add 2.67 g of NH4Cl to the solution.
- Add distilled water until volume is 1 L.
- Quenching solution can be stored at ~20°C for several weeks.
References
“Immunofluorescence Quenching Solution Preparation.” AAT Bioquest, Inc, 02 Jun. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.
