Serological Pipette – Types, Parts, Procedure, Applications

Serological Pipette - Types, Parts, Procedure, Applications

Serological pipette is a long graduated pipette used in laboratory to measure and transfer liquid in ml range. It is commonly used for 1 ml to 50 ml volume transfer. It is mostly disposable plastic (polystyrene) and sometimes glass. It has clear graduation markings on the body to monitor the liquid level during measuring. It … Read more

Pipette (Glass Pipettes) – Principle, Types, Handling, and Uses

Glass Pipettes - Definition, Principle, Types, Handling, and Uses

Pipette (Glass Pipettes) is a laboratory instrument made up of a narrow glass tube that is used to measure and transfer a specific volume of liquid from one container to another. It works by creating a suction or partial vacuum above the liquid (using pipette bulb or mouth suction) so the liquid is drawn inside … Read more

100 Laboratory Equipment Names and Uses

100 Laboratory equipment names and uses

Laboratory equipment is the different tools, instruments and devices used in a laboratory for doing experiments and research work. It is used to measure, observe and handle samples, chemicals and biological specimens in a proper way. It includes measuring instruments, heating and cooling devices, glassware and plasticware, sterilization equipment, microscopes, separation devices, safety equipment and … Read more

Autoclave Validation Methods – Objective, Procedure, Result

Autoclave Validation Methods - Objective, Procedure, Result

Autoclave validation is needed to prove that the autoclave is consistently giving effective sterilization of materials to a required Sterility Assurance Level (SAL). Sterility cannot be guaranteed only by testing the finished product. So, the process has to be validated and documented. Validation is done to check the correct physical parameters like temperature, pressure, and … Read more

Simple Microscope – Definition, Principle, Parts, Uses

Simple Microscope: Working Principle, Uses, Parts, and their Functions.

A simple microscope is an optical device consisting of a single convex lens that magnifies objects by enlarging their image through light refraction. Simple Microscope is a simple optical instrument used to magnify very small objects so that they can be seen by the unaided eye. It is mainly a single convex lens (mostly double … Read more

PH Meter – Principle, Parts, Types, Procedure, Calibration, Application

PH Meter - Principle, Parts, Types, Procedure, Calibration, Application, Examples

pH meter is an instrument used to measure pH of a solution. It measures the hydrogen ion activity in water based solution. It is used to know whether solution is acidic or alkaline. It works on potentiometry principle. It measures the difference in electrical potential between a measuring electrode and a reference electrode. The measuring … Read more

HPLC – Principle, Instrumentation, Types, Uses, Diagram

High-Performance Liquid Chromatography (HPLC)

HPLC (High-performance liquid chromatography) is a liquid chromatography technique used to separate, identify and quantify different components present in a liquid mixture. It is also called high-pressure liquid chromatography. It is used when accurate separation is needed in a mixture. In this technique, a liquid solvent is used as mobile phase and the column is … Read more

Diaphragm of a Microscope – Definition, Types, Mechanism, Functions

Diaphragm of a Microscope - Definition, Types, Mechanism, Functions

Diaphragm of a microscope is a thin opaque disc like part having a circular opening (aperture) which can be adjusted. It is fitted in the light path of microscope, mostly below the stage and near the condenser. It controls the passage of light that will go to the specimen. The main work of diaphragm is … Read more

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis Spectroscopy - Principle, Instrumentation, Applications, Advantages, and Limitation

UV-Vis spectroscopy (Ultraviolet-visible spectroscopy) is an analytical technique used to measure the amount of ultraviolet and visible light absorbed or transmitted by a chemical sample. It measures light in UV and visible region. The reading is taken as absorbance or transmittance. This technique relies on interaction between electromagnetic radiation and matter. When molecules are exposed … Read more

UV Spectroscopy – Principle, Parts, Protocol, Applications

UV Spectroscopy - Principle, Instruments, Applications

UV-Visible (UV-Vis) spectroscopy is an analytical technique used to measure the absorption or transmission of ultraviolet and visible light by a sample. It works in UV region (about 190–400 nm) and visible region (about 400–800 nm). It is a fast and simple method used in both qualitative and quantitative analysis. The absorption is recorded as … Read more