Native Polyacrylamide Gel Electrophoresis (PAGE)

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native PAGE is a gel electrophoresis technique used for separation of proteins and nucleic acids. It is called native because the molecule remains in its natural form. The protein is not unfolded during the process. In this method, SDS, urea and reducing agents are not used. So the protein keeps its original charge, shape and … Read more

Bioreactor – Types, Design, Parts, Applications, Limitations

Bioreactor - Types, Design, Parts, Applications, Limitations

Bioreactor is a special vessel or system used for growing living cells. It is used for microorganisms, plant cells and animal cells. It gives controlled condition for their growth and activity. In bioreactor, temperature, pH, oxygen level and nutrients are controlled properly. Due to this, the cells grow in suitable condition. The metabolic activity of … Read more

Microtome – Principle, Parts, Types, and Uses

Microtome - Principle, Parts, Types, and Uses

Microtome is an instrument used to cut very thin sections of tissues and other materials. These thin sections are used for microscopic examination. The sections are generally about 1 to 100 micrometer in thickness. The word Microtome is derived from Greek words. Mikros means small and temnein means to cut. So microtome means an instrument … Read more

Spectrophotometer Cuvette – Features, Types, Materials, Precautions, Advantages

Spectrophotometer Cuvette - Features, Types, Materials, Precautions, Advantages

Spectrophotometer cuvette is a small transparent tube like laboratory vessel that is used to hold liquid, gas or solid sample during spectroscopic analysis. It is derived from French word cuvette, meaning little vessel. It may be square or circular in cross-section and has straight sides. It has at least two parallel and optically clear windows. … Read more

How to Calibrate ph Meter? Step By step procedure and requirement

How to Calibrate ph Meter?

pH meter is an instrument used to measure the acidity or alkalinity of a solution. It gives the value of pH directly. It is mainly used for aqueous solution. It works like a sensitive voltmeter. It measures the voltage difference produced between two electrodes. One is pH glass electrode and another is reference electrode. The … Read more

Bright Field Microscope – Parts, Principle, Procedure, Application

Bright Field Microscope - Definition, Parts, Working Principle, Application

Bright Field Microscope is a simple optical microscope that uses visible white light to observe the specimen. It is also known as compound light microscope. It is the most commonly used microscope in biology, medicine and laboratory works. In this microscope, light is passed from below the specimen. The light travels through the specimen and … Read more

Ocular Micrometer – Principle, Parts, Procedure, Applications

Ocular Micrometer - Definition, Principle, Parts, Applications

Ocular Micrometer is a precision measuring instrument used in microscope for measuring microscopic objects. It is also known as eyepiece micrometer or reticle. It is used to measure the length, width and diameter of very small objects which are seen under microscope. It is a small circular glass disc which is placed inside the eyepiece … Read more

Toolmakers Microscope – Principle, Procedure, Parts, Applications

Toolmakers Microscope - Principle, Procedure, Parts, Applications

Toolmakers Microscope is a precision optical instrument that is used to measure and inspect the small mechanical components. It is also known as measuring microscope. It is mainly used to observe the shape, size, angle and position of small parts without touching the surface. It consists of an optical head, rigid vertical column, heavy base … Read more

Atomic Absorption Spectrophotometer – Principle, Parts, Types

Atomic Absorption Spectrophotometer

Atomic Absorption Spectrophotometer (AAS) is an analytical instrument used for finding the concentration of metals and metalloids in a sample. It is a sensitive method. It is mostly used when very small amount of element is present in the sample. The instrument works on the absorption of light by free atoms. The atoms must be … Read more