Reverse Transfection: Principle, Protocol, Steps & Applications
Learn what reverse transfection is, its principle, steps and protocol, and how it differs from forward transfection. Includes siRNA, DNA, applications, advantages and limitations.
Learn what reverse transfection is, its principle, steps and protocol, and how it differs from forward transfection. Includes siRNA, DNA, applications, advantages and limitations.
Apoptosis is a regulated cell death process by which unwanted, aged or damaged cells are removed from the body. It is commonly referred to as programmed cell death. This process is important during normal development and maintenance of tissues. During this process, several changes occur in the cell. The cell shrinks. Chromatin becomes condensed and
Learn what plasmids are, how they are structured and replicated, their major types and functions, and how plasmids are used in genetics and biotechnology.
Learn the major plasma membrane models, from Overton and Gorter-Grendel to the Davson-Danielli, Robertson and Singer-Nicolson fluid mosaic models, with diagrams and comparison.
Learn what peripheral proteins are, how they attach to cell membranes, their major functions and examples, and how they differ from integral proteins.
Learn what a channel protein is, how its selective pore enables membrane transport, major channel types, their functions, and how channels differ from carriers.
Learn how the cytoskeleton is built from actin filaments, intermediate filaments, and microtubules and how it controls cell shape, transport, movement, and division.
Learn what microtubules are, how α- and β-tubulin form their structure, why they grow and shrink, and how they support transport, mitosis, cilia, and cell organization.
Learn how intermediate filaments are built, why they are non-polar, where keratins, vimentin, neurofilaments and lamins occur, and how they support cell and tissue strength.
Microfilaments are thin actin filaments of the cytoskeleton. Explore their structure, polarity, dynamics, functions, locations and differences from other cytoskeletal filaments.