Hippurate Hydrolysis Test is a biochemical test used for detecting the enzyme hippuricase(hippurate hydrolase) in microorganism. It is used for checking whether the organism can hydrolyze sodium hippurate or not. This process occurs when bacteria having hippuricase is exposed to sodium hippurate. The substrate is hydrolyzed and two end products is formed. The reaction is … Read more
What is Coliform Presence/Absence Test? Coliform Presence/Absence (P-A) test is a rapid simple and inexpensive qualitative method which is used mainly for monitoring treated drinking water and distribution system for fecal indicator bacteria (coliforms). It is the modification of traditional multiple-tube fermentation technique where a single large water sample volume is inoculated in one culture … Read more
BOD test (Biochemical Oxygen Demand) is a standard laboratory test. It is used for measuring the amount of dissolved oxygen which is consumed by aerobic microorganisms (bacteria) when the organic matter present in water sample is broken down and decomposed. It is the process where oxygen is utilized during biodegradation and this is referred to … Read more
COD Test (Chemical Oxygen Demand test) is a water quality test. It is used to determine the amount of oxygen required to chemically oxidize the organic and inorganic pollutants present in a water sample. It is based on oxidation of pollutants by a strong chemical oxidant. Generally potassium dichromate (K2Cr2O7) is used in heated and … Read more
What is a Glucose Tolerance Test? Glucose Tolerance Test (GTT) is a medical test which is used to measure how effectively the body processes the glucose (sugar) from blood into the tissues. It is most commonly performed as Oral Glucose Tolerance Test (OGTT). It is mainly used for diagnosis of metabolic conditions like prediabetes type … Read more
What is Cell Cycle? Cell cycle is the ordered sequence of events by which a cell grows and divides to form two genetically identical daughter cells. It is the process that ensures continuity of life by producing new cells from pre existing cells. The cell cycle is a carefully regulated process and it occurs in … Read more
What is Checkpoint? A checkpoint is a control point in cell cycle. It is a internal surveillance mechanism present in eukaryotic cell. It ensures that cell division occurs in proper manner. It act like a quality control step where progression is checked before moving to next phase. It is the process where cell checks cell … Read more
Membrane filtration method is a laboratory method which is used for estimation of microbial load (bioburden) in liquid sample like drinking water environmental water and pharmaceutical solution. It is a quantitative method and the bacteria are concentrated on a membrane filter and then counted as colony forming unit (CFU). Principle of this method is based … Read more
What is Heat and Acetic Acid Test? Heat and acetic acid test (heat coagulation test) is a simple qualitative and semi quantitative test which is used for detection of excess protein in urine sample. Mainly albumin is detected but globulin may be present also. It is done as a screening test for proteinuria. Principle of … Read more
Sulfosalicylic acid (SSA) test is a simple screening test which is used to detect excessive proteins in urine. It is a rapid and low cost method and it is mainly used for checking proteinuria which indicates kidney impairment. The test is done on clear urine sample after centrifugation so that turbidity is observed clearly. It … Read more
