Sourav Pan
Mucic acid test is a biochemical test used to detect galactose and galactose containing carbohydrates such as lactose. It is also known as galactaric acid test. In this test, carbohydrate sample is heated with concentrated nitric acid which act as a strong oxidizing agent. The nitric acid oxidizes both aldehyde group and primary alcohol group … Read more
Molisch’s test is a classic chemical test used for detection of carbohydrate in a given sample. It is a highly sensitive test and it is considered as a general screening test for almost all types of carbohydrates. It is named after the Austrian botanist Hans Molisch. This test can detect monosaccharides, disaccharides and polysaccharides. It … Read more
Rapid Furfural Test is a rapid chemical assay used to quickly distinguish between aldohexoses such as glucose and ketohexoses such as fructose. It is done when carbohydrate test solution is mixed with ethanolic α-naphthol solution (Molisch reagent) and concentrated hydrochloric acid (HCl) and then direct boiling is performed. It is based on the principle that … Read more
This test also can differentiate between ketone functional groups and water-soluble carbohydrates.
Solubility Tests is a type of biochemical test which is performed to check the ability of compounds to dissolve within a liquid solvent.
Alkaline Phosphatase (ALP) is an enzyme that is naturally found within all the raw milks that is utilized to determine the quality of pasteurization of milk. A complete pasteurization process can deactivate the enzyme below levels that can be detected by traditional methods. Because the stability of the heat of ALP is higher than the stability of pathogens that could be found in milk, it serves as a metric of security. However, failure for detection of ALP activity is not a mean an item is safe from pathogens.
The carbohydrate fermentation test can be used to determine if bacteria are able to ferment a certain carbohydrate.
It is a test to determine any presence of acids or gas resulting from carbohydrate fermentation.
IMViC test is a group of four biochemical tests which are used in microbiology laboratory to identify and differentiate Gram-negative bacteria especially coliform group and Enterobacteriaceae family. These tests are commonly performed after Gram staining and the reactions are stable and used as important identification tests. IMViC is an acronym in which I stands for … Read more
Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.
Simmons Citrate Agar is a specialized solid microbiological culture medium which is used mainly to differentiate Gram-negative bacteria especially the members of Enterobacteriaceae family. It is used to test the ability of an organism to survive by using citrate as the sole carbon and energy source and ammonium salts as the sole nitrogen source. It … Read more
