What is Algae? Characteristics of Algae Habitat of Algae The Habitat of algae is described as highly diverse, because algae are of universal occurrence and are found in water, land, snow, hot springs, inside plants/animals, and even in extreme saline or aerial conditions. The Aquatic algae are predominant in occurrence, and they are present in … Read more
Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).
What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more
A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.
What is Repetitive sequence-based PCR (rep-PCR)? Working Principle of rep-PCR The rep-PCR working principle is based on the occurrence of conserved repetitive DNA elements (REP, ERIC, BOX, GTG5 etc.), which are dispersed at multiple sites within prokaryotic genomes, and these elements serve as priming sites for amplification. By the use of specifically designed primers, annealing … Read more
What is Nanoparticle-Assisted PCR (nanoPCR)? Working Principle of Nanoparticle-Assisted PCR (nanoPCR) The nanoPCR method is introduced with nanoparticles (NPs) being added into a conventional PCR mixture which contains template DNA, primers, DNA polymerase, dNTPs, and buffer. With optimal NP size (often <100 nm) and concentration being selected, thermal conductivity is enhanced by NPs which accelerates … Read more
Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR 2. High-fidelity PCR 3. Hot-Start PCR 4. Fast-cycling PCR 5. Touch down (TD) PCR 6. Real-Time PCR (qPCR) 7. Digital PCR (dPCR) 8. High-Resolution Melt (HRM) PCR 9. Reverse-Transcriptase PCR (RT-PCR) 10. RT-qPCR – Real-time quantitative reverse transcription … Read more
Microbiology in nursing is the scientific study of microscopic organisms such as bacteria, virus, fungi and protozoa that impact human health in clinical and community care. it deals with how these microbes grow, spread, and survive, also how nurses can prevent or control infections. this knowledge is used daily in patient care, wound dressing, vaccination … Read more
What is cellulose? (C6H10O5)n Cellulose Molecular Weight/ Molar Mass 162.1406 g/mol Density 1.5 g/cm³ Appears White powder Melting Point 260–270 °C Properties of cellulose Structure of cellulose What are cellulases? Cellulases are a group of enzymes that catalyze the hydrolysis of cellulose, a complex polysaccharide present in plant cell walls. These enzymes are largely produced … Read more
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
