SXT (Sulfamethoxazole-Trimethoprim) Test – Principle, Procedure, Result
The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci.
Biochemical Test
Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
Heat Coagulation Test of Proteins – Principles, Procedure, Result
agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.
Millon’s Test – Principle, Procedure, Result, Uses
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
Bial’s Test- Principle, Procedure, Result, Uses
Bial’s test is a chemical test used for detection of pentoses (5-carbon sugars) and it is used to distinguish pentoses from hexoses. It was developed by German physician Manfred Bial. It is also used in laboratory for estimation of RNA because RNA contains pentose sugar ribose. It works on principle of acid dehydration of sugars. … Read more
Beta (β) Lactamase Test – Principle, Procedure, Results
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
Nitrocefin Test (Beta-Lactamase Test) – Principle, Procedure, Uses
The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.
Mucic acid test – Principle, Procedure, Result, Uses
Mucic acid test is a biochemical test used to detect galactose and galactose containing carbohydrates such as lactose. It is also known as galactaric acid test. In this test, carbohydrate sample is heated with concentrated nitric acid which act as a strong oxidizing agent. The nitric acid oxidizes both aldehyde group and primary alcohol group … Read more
Molisch Test – Principle, Procedure, Result, Uses
Molisch’s test is a classic chemical test used for detection of carbohydrate in a given sample. It is a highly sensitive test and it is considered as a general screening test for almost all types of carbohydrates. It is named after the Austrian botanist Hans Molisch. This test can detect monosaccharides, disaccharides and polysaccharides. It … Read more
Rapid Furfural Test for Glucose and Fructose – Principle, Procedure, Result
Rapid Furfural Test is a rapid chemical assay used to quickly distinguish between aldohexoses such as glucose and ketohexoses such as fructose. It is done when carbohydrate test solution is mixed with ethanolic α-naphthol solution (Molisch reagent) and concentrated hydrochloric acid (HCl) and then direct boiling is performed. It is based on the principle that … Read more
Fehling’s Test – Principle, Procedure, Result
This test also can differentiate between ketone functional groups and water-soluble carbohydrates.