Avatar for Sourav Pan

Sourav Pan

Founder of Biology Notes Online, Biology & Microbiology Educator, M.Sc. in Microbiology
Expertise
Microbiology, Immunology, Cell Biology, Molecular Biology, Genetics, Biotechnology, Biochemistry, Botany, Zoology
Education
University of Calcutta
Profile Last Updated: August 10, 2026

Sourav Pan is the founder and sole author of Biology Notes Online. He holds a Bachelor of Science (B.Sc.) degree and an M.Sc. in Microbiology from the University of Calcutta. He creates educational resources covering microbiology, immunology, biotechnology, genetics, cell biology, botany, zoology, and other life-science subjects.

His goal is to make complex biological concepts easier to understand through well-structured, student-friendly articles based on academic textbooks, peer-reviewed research, and authoritative scientific sources. In addition to writing new content, he regularly reviews and updates articles to improve their accuracy, clarity, and educational value.

Transfection PEI (Polyethylenimine) Protocol

Standard protein expression systems, including E. coli, frequently fail to generate folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). Utilizing a eukaryotic system, such as mammalian cells, that includes the required folding and posttranslational machinery is highly advantageous for the expression of these proteins. Using polyethylenimine, we present strategies … Read more

How is plant cell cytokinesis different from animal cell cytokinesis?

How is plant cell cytokinesis different from animal cell cytokinesis?

Cell division is a crucial process that allows organisms to grow, repair, and reproduce. One important aspect of cell division is cytokinesis, the process of separating one cell into two daughter cells. While cytokinesis is a universal process, it can vary greatly between different types of cells. In this article, we will compare and contrast … Read more

Phytoremediation – Definition, Types, Mechanisms

Phytoremediation - Definition, Types, Mechanisms

What is Phytoremediation? Types of Phytoremediation There are three varieties of phytoremediation: 1. In situ phytoremediation Advantages of In situ phytoremediation Disadvantages of In situ phytoremediation 2. In-vivo phytoremediation with relocated contaminants 3. In vitro phytoremediation Processes Of Phytoremediation Phytoremediation is based on certain natural processes carried out by plants, such as:  Mechanisms of phytoremediation … Read more

Parasitism – Definition, Types, Examples

Parasitism - Definition, Types, Examples

What is Parasitism? Definition of Parasitism Parasitism is a symbiotic relationship between two species in which one organism, the parasite, derives benefits at the expense of the other organism, the host, often causing it harm. Mechanisms of Dispersal Types of Parasitism  Parasitism, a multifaceted biological interaction, can be classified based on various criteria, ranging from … Read more

Biotic Factors – Definition, Types, Examples

Biotic Factors - Definition, Types, Examples

Definition of Biotic Factor  Biotic factors are the living components or variables that have an effect on an ecosystem or other species residing in that ecosystem. Biology definition A biotic factor is a factor produced by a live organism or any living component in its surroundings as a result of the organism’s actions. Biotic factors … Read more

Bioaccumulation – Definition, Mechanisms, Applications

Bioaccumulation - Definition, Mechanisms, Applications

What is Bioaccumulation? Definitions of Bioaccumulation Bioaccumulation (increase in chemical concentration) is the process of chemical accumulation in an organism over time. Bioaccumulation, as defined by the International Union of Pure and Applied Chemistry, is the accumulation of a chemical in an organism due to direct uptake from the environmental matrix (bioconcentration) and uptake from … Read more

Transfection Protocols – Principle, Protocol, Uses

Transfection Protocols - Principle, Protocol, Uses

Principle of Transfection According to the definition of transfection, the external genetic material must enter the cell via the cell membrane. It is essential to highlight that the genetic material, whether DNA or RNA, is negatively charged as a result of the proteins that surround it. Therefore, in an unmanipulated environment, exogenous DNA cannot cross … Read more

Bioaerosol – Definition, Types, Collection, Transmission

Bioaerosol - Definition, Types, Collection, Transmission

What is Bioaerosol? Types of bioaerosols 1. Fungi 2. Bacteria 3. Viruses 4. Pollen Collection Methods of Bioaerosol/Deposition of Bioaerosol Bioaerosols are primarily collected via collection plates, electrostatic collectors, mass spectrometers, and impactors. Other experimental approaches are also utilised. In comparison to other PC filter alternatives, Polycarbonate (PC) filters have produced the most accurate bacterial … Read more

Biological Oxygen Demand (BOD) – Definition, Importance, Measurement

Biological Oxygen Demand (BOD) Definition, Importance, Measurement

Biological oxygen demand refers to the amount of oxygen required by bacteria and other microorganisms to decompose organic matter under aerobic (oxygen present) conditions at a certain temperature (BOD). The greater the concentration of organic contaminants in the water, the greater the oxygen demand of the bacteria. Consequently, the level of contamination in a body … Read more

Consumers – Definition, Types, Examples

Consumers - Definition, Types, Examples

What is Consumer? Consumer is a category that belongs inside an ecosystem’s food chain. It primarily refers to animals. Consumers cannot generate their own energy and must rely on the intake and digestion of producers, other consumers, or both in order to survive. Types of consumer 1. Primary consumers Examples of Primary consumers 2. Secondary … Read more