Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.
Cell membrane staining is the process in which the plasma membrane of a cell is visualized with the help of dyes or fluorescent probes. It is the thin outer covering of the cell, and it is normally transparent, so it cannot be seen clearly under a simple light microscope. It is the process where special … Read more
Nuclear staining is the process in which the nucleus of a cell is given artificial colour so that it becomes visible under microscope. It is the main method used in histology and cytology because the nucleus is naturally transparent and it needs specific dyes for proper contrast. The nucleus contains DNA and chromatin materials which … Read more
The composition of cell wall varies from species to species, it has been reported that the main constituents of cell wall is chitin, hemicellulose, and cellulose. In addition, prokaryotic cell wall contains peptidoglycan (also known as murein and mucopeptide). Peptidoglycan is mainly composed of sugar, amino acids (peptide; amino acids + glycan; sugar).
Cell viability analysis using Propidium Iodide is a simple method in which the dye is used to distinguish between living and dead cells. It is the process where Propidium Iodide (PI) is added to a cell suspension and the stained cells is then examined usually by flow cytometer. PI is a red-fluorescent dye that binds … Read more
Loeffler’s methylene blue staining is a simple staining method used mainly for observing the morphology of Corynebacterium diphtheriae. It is the process where Loeffler’s alkaline methylene blue solution is applied on a fixed smear, and this stain contains a small amount of potassium hydroxide (KOH) which makes the solution alkaline. It is the alkalinity that … Read more
Giemsa stain is a polychromatic nucleic acid stain that is used mainly for demonstrating different blood cells and parasites in stained blood films. It is named after the German chemist Gustav Giemsa. It belongs to a group of stains known as Romanowsky stains. These are neutral stains made from a mixture of oxidized methylene blue, … Read more
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
