Eastern blot – Protocol, Principle, Application
A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.
Practical
Subcategories
Dot Blot – Protocol, Principle, Definition.
Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
Restriction Digest – Protocol, Principle, result
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
Reverse Blood Grouping – Principle, Procedure, Result
Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.
Catalase Test Principle, Procedure, Result and Application.
The catalase test is a type of biochemical test which is used to detect the production of catalase enzymes in the organism.
Xanthoproteic test – Principle, Procedure, Result, Application
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc. The presence of proteins can be confirmed qualitatively by usign several tests, Xanthoproteic test is one of them. Due to the presence of characteristic side chains in them, certain amino acids exhibit typical color reactions that form the basis for their identification.
Modified Kirby-Bauer Disc Diffusion Method
Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory.
Myeloperoxidase (MPO) Stain – Principle, Procedure, Result
Myeloperoxidase is a lysosomal enzyme that can be found in the azurophil granules of neutrophils and monocytes.
Yeast Under the Microscope
What is Yeast? Yeast are tiny, single-celled fungi that play a crucial role in creating everyday delights like bread, beer, and wine through a process called fermentation. These minuscule organisms have an impressive ability to multiply and adapt, making them essential for various culinary and scientific applications. These unicellular organisms, belonging to the Fungus Kingdom, … Read more
Anabaena Under Microscope
What is Anabaena? Requirement for Observation of Anabaena Under Microscope Procedure for observation of Anabaena Under Microscope This procedure outlines the steps for creating a wet mount slide and observing the prokaryotic alga Anabaena under a microscope. Structured Inquiry Step 1: Estimating Field of View Begin by placing a clear millimeter ruler on the microscope … Read more