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Nanoparticle-Assisted PCR (nanoPCR) – Definition, Principle, Steps, Applications

• • 10 min readby
Nanoparticle-Assisted PCR (nanoPCR)

What is Nanoparticle-Assisted PCR (nanoPCR)? Working Principle of Nanoparticle-Assisted PCR (nanoPCR) The nanoPCR method is introduced with nanoparticles (NPs) being added into a conventional PCR mixture which contains template DNA, primers, DNA polymerase, dNTPs, and buffer. With optimal NP size (often <100 nm) and concentration being selected, thermal conductivity is enhanced by NPs which accelerates … Read more

Types of PCR with Definition, Principle, and Uses

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Types of PCR with Definition, Principle, and Uses

Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR 2. High-fidelity PCR 3. Hot-Start PCR 4. Fast-cycling PCR 5. Touch down (TD) PCR 6. Real-Time PCR (qPCR) 7. Digital PCR (dPCR) 8. High-Resolution Melt (HRM) PCR 9. Reverse-Transcriptase PCR (RT-PCR) 10. RT-qPCR – Real-time quantitative reverse transcription … Read more

Western Blot – Steps, Principle, Result, Applications

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Western Blotting Experiment

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.

Brucella Agar – Composition, Principle, Preparation, Results, Uses

• Edited by Sourav Pan • • 0 revisions • 14 min readby
Brucella Agar - Composition, Principle, Preparation, Results, Uses

Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.

Southern Blot – Definition, Principle, Steps, Importance

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Southern Blotting Principle, Procedure, Importance

Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.

PCR Machine – Principle, Parts, Types Steps, Applications

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PCR Machine - Definition, Principle, Parts, Types Steps, Applications, Examples

A PCR machine is a common tool found in labs, especially if you’ve ever dealt with DNA testing or medical diagnostics. At its core, it’s designed to create millions of copies of a specific DNA segment, which sounds simple but is incredibly powerful for tasks like identifying infections, studying genetic mutations, or even solving crimes. … Read more

Electroporator – Definition, Principle, Types, Protocol, Applications

• • 20 min readby
Electroporator – Definition, Principle, Types, Protocol, Applications

What is Electroporator (Electroporation machine)? An electroporator, often called an electroporation machine, is a lab instrument used to temporarily make cell membranes more permeable. Think of it as a precision tool that helps scientists sneak substances like DNA, drugs, or proteins into cells—something that’s tricky to do manually because cell membranes are naturally protective. The … Read more

Micropipettes – Parts, Types, Applications, Techniques

• • 30 min readby
Micropipettes – Parts, Types, Applications, Techniques

What is a micropipette? A micropipette is a precision laboratory instrument designed to measure and transfer extremely small volumes of liquid, typically ranging from 0.1 microliters to 1,000 microliters. Commonly used in molecular biology, chemistry, and medical labs, it operates like a highly accurate eyedropper, enabling researchers to handle samples with meticulous control. The device … Read more

Column Chromatography – Principle, Steps, Parts, Uses, Advantages

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Column Chromatography - Procedure, Instruments, Application, Advantages

What is Chromatography? Chromatography is a powerful laboratory method used to separate, identify, and analyze the components of complex mixtures. Think of it as a molecular race where different substances travel at varying speeds based on their interactions with two key elements: a stationary phase (a solid or liquid-coated surface) and a mobile phase (a … Read more

Spectrophotometer – Principle, Parts, Types, Mechanism, Uses

• • 22 min readby
Spectrophotometer - Principle, Parts, Types, Mechanism, Uses

What is a Spectrophotometer? Definition of Spectrophotometer A spectrophotometer is a laboratory instrument that measures the amount of light absorbed by a sample and is used to determine properties such as concentration or absorption patterns of substances. Principle of Spectrophotometer Types of Spectrophotometer Spectrophotometers are analytical devices for measuring, at designated wavelengths, the intensity of … Read more