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A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.
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What is Microprojectile Bombardment? Definition of Microprojectile Bombardment Microprojectile bombardment, also known as biolistics or gene gun delivery, is a method used to introduce foreign genetic material into cells or tissues by accelerating microscopic particles coated with the desired DNA, RNA, or proteins at high velocities, facilitating direct gene transfer. This technique is commonly employed … Read more
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What is gene expression? Gene expression definition Gene expression is the process by which the genetic information encoded in a gene is used to produce a functional product, typically a protein or a specific RNA molecule. Stages in Gene Expression 1. Transcription 2. Translation What is gene expression profiling? Methods or Steps of gene expression … Read more
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Post-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an primary RNA transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell.
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What is Restriction Enzyme (Restriction Endonuclease)? Definition of Restriction Enzyme (Restriction Endonuclease) A restriction enzyme, also known as a restriction endonuclease, is an enzyme that cuts DNA at specific recognition sites, called restriction sites. It is primarily found in bacteria and archaea and serves as a defense mechanism against invading viruses. Restriction enzymes are commonly … Read more
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What is RNA? Different Types of RNA and Their Functions RNA, or Ribonucleic acid, is a crucial molecule synthesized by RNA polymerase from DNA. It serves various functions in the cell, including protein-coding and non-coding roles. Understanding the different types of RNA is essential for comprehending their specific functions. Let’s delve into the main types … Read more
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Pulsed Field Gel Electrophoresis (PFGE) is a highly effective genotyping technique used for the separation and analysis of large DNA molecules, such as entire genomic DNA. It involves digesting the DNA with specific restriction enzymes and applying it to a gel matrix under an electric field that periodically changes direction. PFGE is a modified version … Read more
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What is DNA Sequencing? Definition of DNA Sequencing DNA sequencing refers to the techniques used to determine the order of the nucleotide bases adenine, guanine, cytosine, and thymine within a DNA molecule. Methods of DNA Sequencing DNA sequencing is a crucial technique used in various fields of biological research, allowing scientists to unravel the genetic … Read more
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The DNA microarray technology is among the most effective technology that can offer an extremely high-throughput and precise overview of the whole transcriptome and genome, which lets scientists understand the molecular processes that underlie natural and malfunctioning biological processes. Microarray technology may accelerate the process of screening thousands of protein and DNA samples at once.
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Protein synthesis regulation is the intricate process by which cells control the production of proteins, which are essential for nearly all biological functions. This process involves the precise regulation of gene expression to ensure the synthesis of specific proteins at the right time and in the right amounts. Protein synthesis regulation plays a vital role … Read more