Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

Synchronous Culture – Definition, Methods, Application.

Synchronous culture Definition

A synchronous or synchronized culture is a microbiological culture or a cell culture which contains the cells that are all in the same growth stage.

  • The Synchronous culture also known as the  synchronous growth.
  • The synchronous growth of a bacterial culture means all the cells of the culture remain at the same stage of growth and they grow all together from one phase to another.
  • A population can be synchronized by manipulating their physical environments or physical composition of the medium.
  • If we keep an organism under unfavourable conditions there they will metabolize very slowly, but will not divide. If we keep the organisms under favourable conditions, then all cells undergo division and stay at the same phase.
  • The cells of the synchronously growing culture divide at a time, their growth curve forms a Zig Zag pattern.
  • The easiest way to synchronize bacterial growth is to add some cytostatic agents so that cells don’t divide and they all maintain the same state of metabolism and cell cycle. When the cytostatic agent is removed, all cells start to divide at the same time.
  • Synchronous culture/Synchronous growth of bacteria consists of two phases such as stationary phase and exponential phase.
Synchronous culture curve
Synchronous culture curve

Obtaining Synchronous culture 

There are present different method for obtaining a Synchronous culture such as;

  • To arrest the growth of all cells in the culture, alter the External conditions, and then again alter the conditions to continue the growth. As a result of this, all the fresh growing cells are now beginning to grow at the same stage, and they are synchronized.
  • Synchronous culture can be obtained by eliminating the essential nutrient from the growth medium and later re-introduce it.
  • The chemical growth inhibitors can be used to stop cell growth. When the growth is completely stopped for all cells, then remove the inhibitor from the culture and the cells will begin to grow synchronously. For example, Nocodazole is used in biological research for synchronization.
  • Cells in various growth stages contain distinct physical properties. Cells in culture can thus be physically divided depending on their density or size, for instance. This can be done by using centrifugation (for density) or filtration (for size).
  • The Helmstetter-Cummings technique can be used to obtain a Synchronous culture. In this method, the bacterial culture is passed through a membrane. Some of these cells will remain bound to the membrane. After that a fresh medium is added to the membrane, as result, the membrane-bound bacteria will start to grow. Fresh bacteria that separated from the membrane are now all at the identical stage of growth; they are accumulated in a flask that now harbors as a synchronous culture.

Application of Synchronous culture

  • Synchronous culture helps in the separation of the smallest cells from an exponentially growing culture. 
  • In the laboratory it is used to study the cell cycle.
  • Important in the study of genetics and metabolism.

References

  • https://www.slideshare.net/SivaSss1/bacterial-growth-diauxic-growthsynchronous-growth-and-continuous-growth
  • https://en.wikipedia.org/wiki/Synchronous_culture
  • https://www.mybiosource.com/learn/testing-procedures/synchronous-cultures-micro-organisms/

Related Posts

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.

What is Karyotyping? What are the scope of Microbiology? What is DNA Library? What is Simple Staining? What is Negative Staining? What is Western Blot? What are Transgenic Plants? Breakthrough Discovery: Crystal Cells in Fruit Flies Key to Oxygen Transport What is Northern Blotting? What is Southern Blotting?
What is Karyotyping? What are the scope of Microbiology? What is DNA Library? What is Simple Staining? What is Negative Staining? What is Western Blot? What are Transgenic Plants? Breakthrough Discovery: Crystal Cells in Fruit Flies Key to Oxygen Transport What is Northern Blotting? What is Southern Blotting?
Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×