Red Blood Cell – Morphology, Size, Shape, Color and Inclusion Bodies

Red Blood Cell Morphology: Size, Shape, Color and Inclusion Bodies

Red blood cells constitute the primary cellular component in blood. Red blood cells that are mature are biconcave discs which have no nucleus and are devoid of most cell organelles , including the lysomes, endoplasmic-reticulum and mitochondria.

Types of Plant Cell

Types of Plant Cell

They are multicellular, eukaryotic cells which comprise the Plant (a collection of eukaryotes that belong to the Plantae kingdom ) capable of producing their own food from sunlight, water and carbon dioxide). Since they are eukaryotic they possess a distinct nucleus, with specially designed organelles that allow them to function in a controlled way.

Cell Growth – Definition, Types, Mechanisms

Cell Growth - Definition, Types, Mechanisms

Growth Definition Levels of Growth  There are two distinct stages of growth in living organisms: A. Cell growth B. Growth of multicellular organisms The growth of multicellular animals and plants in connection to the growth and multiplication of their individual cells can be divided into three distinct categories: (1) Auxetic growth (Auxesis = growth resulting … Read more

Genetic Code – Definition, Characteristics, Wobble Hypothesis

Genetic Code - Definition, Characteristics, Wobble Hypothesis

What is a Genetic Code? The genetic code is a set of rules that living cells use to decipher the information encoded in genetic material (DNA or mRNA sequences). The ribosomes are responsible for carrying out the translation process. Using tRNA (transfer RNA) molecules to carry amino acids and to read the mRNA three nucleotides … Read more

Nucleolus – Definition, Structure, Functions

Nucleolus - Definition, Structure, Functions

The nucleolus is a structure within a cell’s nucleus that plays a critical role in the life of a cell. This small but mighty organelle is responsible for several important functions, including the synthesis and assembly of ribosomes. In this article, we will explore the nucleolus, its structure and function, and its role in maintaining … Read more

Transfection Protocols – Principle, Protocol, Uses

Transfection Protocols - Principle, Protocol, Uses

Principle of Transfection According to the definition of transfection, the external genetic material must enter the cell via the cell membrane. It is essential to highlight that the genetic material, whether DNA or RNA, is negatively charged as a result of the proteins that surround it. Therefore, in an unmanipulated environment, exogenous DNA cannot cross … Read more

Transfection PEI (Polyethylenimine) Protocol

Transfection PEI (Polyethylenimine) Protocol

Standard protein expression systems, including E. coli, frequently fail to generate folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). Utilizing a eukaryotic system, such as mammalian cells, that includes the required folding and posttranslational machinery is highly advantageous for the expression of these proteins. Using polyethylenimine, we present strategies … Read more

Lentiviral Transfection Protocol

Lentiviral Transfection Protocol

Lentiviral transfection is a method for introducing genetic material into cells. It involves the use of lentiviruses, a type of retrovirus, to deliver the desired DNA into the target cells. Lentiviral transfection has a unique advantage over other transfection methods, in that it can infect both dividing and non-dividing cells, making it useful for a … Read more

Reverse transfection – Definition, Protocol, Applications

Reverse transfection - Definition, Protocol, Applications

Reverse transfection is a technique used in molecular biology to introduce a sample of RNA, DNA or protein into cells in culture. Instead of adding the reagents to the cells and allowing them to take them up (forward transfection), reverse transfection involves incubating the cells on a surface that is coated with the reagents. The … Read more

Apoptosis Pathway- Definition, Functions, Mechanism, Examples

Apoptosis Pathway- Definition, Functions, Mechanism, Examples

Learn all about the apoptosis pathway: its definition, functions, mechanism, and examples in this comprehensive guide. Apoptosis, also known as programmed cell death, is a crucial process for maintaining healthy cell turnover and eliminating damaged or abnormal cells. Understanding the various components and steps involved in the apoptosis pathway can provide valuable insights into diseases … Read more

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