Microbiology

Direct Microscopic Count (DMC) Cell Counting Formula In Counting Chamber The number of bacterial cell per milliliter (ml) = Number of cells counted X dilution (if used) X 50,000* The factor of 50,000 is used in order to determine the cell count for 1 ml: 1 ml = 1000 mm3 = (50 times the chamber … Read more

Binomial nomenclature means a system that is used to provide a unique name to organisms, including plants, animals, microorganisms, and other living things. This naming system makes it easier to understand them better and also separates each species from others. Therefore a universally accepted naming system is essential. Binomial nomenclature definition Binomial nomenclature is a … Read more

What is Carl Woese’s Classification? Carl Woese’s Classification Why is rRNA a good ‘Chronometer’? Ribosomal RNA (rRNA) has been recognized as a pivotal molecular tool in the realm of phylogenetic studies and evolutionary biology. Its efficacy as a ‘chronometer’ can be attributed to several intrinsic properties that make it an ideal candidate for tracing evolutionary … Read more

The food supply upon which microorganisms thrive in laboratories is termed as a culture medium or culture media. Culture Medium Definition The nutrient preparation on or in which a culture (microorganism) is grown in the laboratory is known as culture medium or culture media. Classification of culture media/Types of Culture Medium Many special-purpose media are … Read more

In this article, we will learn about the Membrane filtration methods, definition, applications, advantages, disadvantages. Before heading towards Membrane Filtration, you have to understand what is filtration? Membrane filtration is a method of separating particles or molecules from a liquid or gas stream using a semi-permeable membrane. The membrane acts as a barrier, allowing some … Read more

Continuous Culture Definition Continuous Culture is an ‘open’-culture system for the cultivation of microorganisms or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. Continuous Culture Advantages System applied for Continuous Culture There are two systems used for Continuous Culture … Read more

Synchronous culture Definition A synchronous or synchronized culture is a microbiological culture or a cell culture which contains the cells that are all in the same growth stage. Obtaining Synchronous culture  There are present different method for obtaining a Synchronous culture such as; Application of Synchronous culture References

What is Diauxic growth? When a bacterium is incubated in a medium containing two sugars one monosaccharide and another di or polysaccharide (like glucose and lactose). The bacteria first utilize glucose and complete their lag and log phase. After completion of glucose utilization, they used lactose and produce and another lag phase due to the … Read more

Microbial Control Methods Types of Microbial Control Methods There are present four distinct methods which are used to control the growth of microbes such as; Different terms used in Microbial Control Methods The following terms are used to describe the processes and chemical agents employed in controlling microorganisms. What is Sterilization? What is Disinfection? What … Read more

Chemical Method of sterilization Characteristics of an ideal Chemical Antimicrobial agent or disinfectant A chemical antimicrobial agent should contain the following characteristics; How to Select An Ideal Chemical Agent for Practical Application? There are three major factors that need to be assessed in the process of selecting the most appropriate chemical agent for a specific … Read more