Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides.
The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci.
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
Bial’s test is a chemical test used for detection of pentoses (5-carbon sugars) and it is used to distinguish pentoses from hexoses. It was developed by German physician Manfred Bial. It is also used in laboratory for estimation of RNA because RNA contains pentose sugar ribose. It works on principle of acid dehydration of sugars. … Read more
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.
Mucic acid test is a biochemical test used to detect galactose and galactose containing carbohydrates such as lactose. It is also known as galactaric acid test. In this test, carbohydrate sample is heated with concentrated nitric acid which act as a strong oxidizing agent. The nitric acid oxidizes both aldehyde group and primary alcohol group … Read more
Molisch’s test is a classic chemical test used for detection of carbohydrate in a given sample. It is a highly sensitive test and it is considered as a general screening test for almost all types of carbohydrates. It is named after the Austrian botanist Hans Molisch. This test can detect monosaccharides, disaccharides and polysaccharides. It … Read more
Rapid Furfural Test is a rapid chemical assay used to quickly distinguish between aldohexoses such as glucose and ketohexoses such as fructose. It is done when carbohydrate test solution is mixed with ethanolic α-naphthol solution (Molisch reagent) and concentrated hydrochloric acid (HCl) and then direct boiling is performed. It is based on the principle that … Read more
This test also can differentiate between ketone functional groups and water-soluble carbohydrates.
