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Total Red Blood Cell (RBC) Count – Procedure, Principle, result

Total Red Blood Cell (RBC) Count - Procedure, Principle, Result

Haemocytometry is a quantitative method that quantifies the RBC count in blood samples. The manual method of counting RBCs is the Haemocytometer (or Neubauer’s chamber slides). Modern devices, such as photometric and electrometric counters, can be more precise or automated. They can count the cells in the blood sample. It is impossible to count the … Read more

Sahli’s Method For The Estimation Of Hemoglobin

Sahli’s Method For The Estimation Of Hemoglobin

Hemoglobin, often abbreviated as Hb, is a vital protein found in red blood cells (RBCs). Its primary role in the human body includes the transport of oxygen from the lungs to various tissues and the transfer of carbon dioxide from tissues back to the lungs. Additionally, hemoglobin plays a significant role in maintaining the pH … Read more

Blood Cross Matching – Principle, Procedure, Result.

Cross Match

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Blood Grouping Test – Principle, Procedure, Result

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Gel Staining Procedure for PAGE

Gel Staining Procedure for PAGE

Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.

Laboratory Rules – Basic Rules Of A Microbiology Laboratory

Laboratory Rules: Basic Rules Of A Microbiology Laboratory

A well-established microbiology laboratory is necessary for the work of microorganisms. The students/researchers/scientists must follow the basic rules while working with microorganisms.

Measurement Of Microorganisms – Micrometry

Measurement Of Microorganisms - Micrometry

A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.

Giolitti-Cantoni Broth for Staphylococcus aureus

Giolitti-Cantoni Broth for Staphylococcus aureus

Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.

Eastern blot – Protocol, Principle, Application

Eastern blot Protocol

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

Methylene Blue Reduction Test

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Methylene Blue Reduction Test also known as mbrt test. It is a qualitative test for milk, it used to check the quality of raw and pasteurized milk. The Methylene Blue Reduction Test is based on the fact that in the presence of oxygen the methylene blue solution forms blue color, and it will lose the … Read more

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