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Lipase Test – Principle, Procedure, results

Lipase Test Principle, Procedure, results

What is a lipase test? Lipase is a digestive enzyme that might be thought of as “digestive juice.” It aids in the breakdown of fatty tissue. To produce lipase, your body relies primarily on the pancreas, an organ situated behind and below the stomach. Salivary (spit) glands and the stomach both produce lipase. A trace … Read more

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).

Bile Solubility Test – Principle, Procedure, Result

Bile Solubility Test - Principle, Procedure, Result

What is Bile Solubility Test? Definition of Bile Solubility Test The bile solubility test is a biochemical assay used to differentiate Streptococcus pneumoniae from other alpha-hemolytic Streptococci based on the lysis of bacterial cells in the presence of specific bile salts. Objectives of Bile Solubility Test In essence, the bile solubility test is a pivotal … Read more

OF (Oxidation-Fermentation) Test Principle, Purpose, Procedure

OF (Oxidation-Fermentation) Test Principle, Purpose, Procedure

Purpose of OF Test Principle of OF Test Hugh and Leifson’s OF basal medium Composition of Hugh and Leifson’s OF basal medium Ingredients g/l Peptone (tryptone) 2.0 g Sodium chloride 5.0 g Glucose (or other carbohydrate) 10.0 g Bromthymol blue 0.03 g Agar 3.0 g Dipotassium phosphate 0.30 g Preparation Procedure of OF (Oxidation-Fermentation) Test … Read more

Novobiocin Susceptibility Test Principle, Procedure, Results

Novobiocin Susceptibility Test Principle, Procedure, Results

The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

What is Photosystem 1 (PS1)? What is Photosystem 2 (PS2)? Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2) Feature Photosystem I (PSI) Photosystem II (PSII) Location in Thylakoid Membrane Outer surface Inner surface Photocenter P700 P680 Absorbing Wavelength >680 nm <680 nm Photophosphorylation Both cyclic and non-cyclic Only non-cyclic Photolysis of Water No Yes … Read more

Top 15 Books for Molecular Biology

Top 15 Books for Molecular Biology

In this article, I will share a list of Top and Best Molecular Biology Books. In this paper on microbiology, you will study about Structures of DNA and RNA / Genetic Material,  Replication of DNA (Prokaryotes and Eukaryotes), Transcription in Prokaryotes and Eukaryotes, Post-Transcriptional Processing, Translation (Prokaryotes and Eukaryotes), Regulation of gene Expression in Prokaryotes … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more

Centrifuge Blood Sample – Purpose, Steps, Techniques

Centrifuge Blood Sample - Purpose, Steps, Techniques

What are the components of blood? Blood is a fluid tissue that circulates throughout the body and performs a number of important functions. It is made up of several components, including cells and plasma. Cells: Plasma: Plasma is the liquid portion of the blood that carries the cells and other substances throughout the body. It … Read more

Reticulocyte Count – Principle, Procedure, Calculations and Clinical Significances

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have not yet completed the final stages of maturation. They are usually produced in the bone marrow and have a characteristic reticulated (network-like) appearance when stained with special dyes. Reticulocytes are important in the evaluation of anemia, as they provide information about the bone … Read more

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