Dot Blot – Protocol, Principle, Definition.
Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
Restriction Digest – Protocol, Principle, result
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
Reverse Blood Grouping – Principle, Procedure, Result
Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.
Catalase Test Principle, Procedure, Result and Application.
The catalase test is a type of biochemical test which is used to detect the production of catalase enzymes in the organism.
Xanthoproteic test – Principle, Procedure, Result, Application
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc. The presence of proteins can be confirmed qualitatively by usign several tests, Xanthoproteic test is one of them. Due to the presence of characteristic side chains in them, certain amino acids exhibit typical color reactions that form the basis for their identification.
Modified Kirby-Bauer Disc Diffusion Method
Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory.
Myeloperoxidase (MPO) Stain – Principle, Procedure, Result
Myeloperoxidase is a lysosomal enzyme that can be found in the azurophil granules of neutrophils and monocytes.
Herpes simplex virus 1 (HSV-1)
Herpes simplex viruses (HSVs) are highly host-adapted viruses that can cause a variety of diseases in human hosts. (a) herpes simplex virus type 1 (HSV-1) and (b) herpes simplex virus type 2 (HSV-2) are the two forms of HSVs (HSV-2). Both types share DNA homology, antigenic determinants, tissue tropism, and illness symptoms, but their epidemiology … Read more
Influenza A Virus – Structure, Genome, Replication, Treatment, Prevention
What is Influenza Virus? Classification of Influenza Virus Influenza viruses are often classified into three species: influenza A, influenza B, and influenza C. Only types A and B are relevant to human health. Despite the fact that both viruses are capable of infecting humans, genus A often causes widespread disease and epidemics. Influenza A Virus … Read more
Polio Virus – Structure, Genome, Replicationm, Pathogenesis
Polio Virus Structure of Polio Virus In summary, the poliovirus is a small, simple virus that contains a naked RNA genome enclosed in a protein shell. The capsid is composed of four structural proteins that form the basic building blocks of the capsid. The virus does not have a lipid envelope and is insensitive to … Read more