Sudan Black B Staining – Principle, Procedure, Uses
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.
Biology Practicals
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
Flagella Staining – Principle, Procedure, Result
Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has been used for visualization of flagella is known as wet mount technique.
Leifson Technique for Bacterial Flagella Staining
In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from flagellated bacteria were common, as well as mutation leading to loss of motility without loss of the flagellum itself.
Thick and Thin Blood Smear Preparation
A blood smear is a simple diagnostic preparation where a drop of peripheral blood is spread on a clean slide, and it is dried for staining. It is the process used for observing blood cells and also for detecting parasites after staining methods like Giemsa. It is an important technique because it helps in demonstrating … Read more
Hanging Drop Method (Motility test) – Principle, and Procedure, Result, Uses
Hanging drop technique allows for the analysis of living microorganisms. This involves fixing the Microbial suspension into a drop of liquid over the slide of glass.
Bacterial Smear – Principle, Preparation Steps, Uses, Limitations
A bacterial smear is a thin layer of bacterial cell/culture which is placed on a clean, grease-free microscopic slide to observe a clear image under a microscope.
Latex agglutination test – Definition, Procedure, Principle, Advantages, Limitation, Uses
Latex agglutination test is a simple serological test where antigen and antibody reaction is made visible on the surface of latex particles. It is the process in which latex beads are coated either with antigen or with antibody and when a sample containing the corresponding component is added, the beads is clumped together forming visible … Read more
Temporary Wet Mount technique – Procedure, Requirement, Uses
In Mounting technique, the specimen to be observed is held on a microscopic slide by using a coverslip.
Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs
Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.