Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
Culture medium refers to the nutrient-rich substance that is used in labs to culture microorganisms such as bacteria and fungi. These materials, whether liquid or solid, provide the microbes with needed nutrients, minerals and other factors for growth and reproduction according to step-by-step reports from authoritative sources around the world. The late 19th century was … Read more
Culture Media Examples Components/Comments Primary Purpose Bile esculin agar (BEA) Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria. Differential isolation and presumptive identification of group D streptococci and enterococci Bile esculin azide agar with vancomycin Contains azide to inhibit … Read more
When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.
What is Flame Photometer? Principle of Flame photometer The flame photometer is based on the principle that alkali and alkaline earth metal atoms are excited in the flame and that the light emitted when the excited atoms revert to the ground state can be measured. When you introduce a sample containing metal compounds into a … Read more
What are animal and plant cells? Animal and plant cells serve as the building blocks of life for animals and plants, respectively. Both are eukaryotic cells, meaning they contain a true nucleus and specialized organelles, but they also display significant structural and functional differences. Animal Cells Animal cells are generally smaller and have a flexible, … Read more
Materials required The study of legumes, particularly in understanding their biochemical composition and properties, requires specific materials to conduct experiments effectively. The following items are essential for analyzing legumes such as Glycine max (soybean) and Cicer arietinum (gram). Each material plays a critical role in the experimental process, ensuring accurate results and insights into the … Read more
