Standard protein expression systems, including E. coli, frequently fail to generate folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). Utilizing a eukaryotic system, such as mammalian cells, that includes the required folding and posttranslational machinery is highly advantageous for the expression of these proteins. Using polyethylenimine, we present strategies … Read more
Lentiviral transfection is a method for introducing genetic material into cells. It involves the use of lentiviruses, a type of retrovirus, to deliver the desired DNA into the target cells. Lentiviral transfection has a unique advantage over other transfection methods, in that it can infect both dividing and non-dividing cells, making it useful for a … Read more
Reverse transfection is a technique used in molecular biology to introduce a sample of RNA, DNA or protein into cells in culture. Instead of adding the reagents to the cells and allowing them to take them up (forward transfection), reverse transfection involves incubating the cells on a surface that is coated with the reagents. The … Read more
Learn all about the apoptosis pathway: its definition, functions, mechanism, and examples in this comprehensive guide. Apoptosis, also known as programmed cell death, is a crucial process for maintaining healthy cell turnover and eliminating damaged or abnormal cells. Understanding the various components and steps involved in the apoptosis pathway can provide valuable insights into diseases … Read more
What are Plasmids? Definition of Plasmids Plasmids are small, circular, extrachromosomal DNA molecules found primarily in bacteria that can replicate independently of the main chromosomal DNA and often carry genes beneficial to the host organism, such as those conferring antibiotic resistance. Properties/Characteristics of Plasmids Bacterial plasmids are extrachromosomal DNA fragments that exist within bacterial cells. … Read more
Plasma Membrane Functions of Plasma Membrane Models of Plasma Membrane Following are descriptions of the top four historical Plasma Membrane models. The examples are: 1. Lipid and Lipid Bilayer Model 2. Unit Membrane Model (Protein-Lipid Bilayer-Protein) 3. Fluid Mosaic Model Modifications of Fluid Mosaic Model 1. Microvilli 2. Intercellular Spaces 3. Interdigitations 4. Desmosomes and … Read more
What is Peripheral Protein? Structure of Peripheral Proteins Several peripheral proteins are marked in the picture below. A peripheral protein doesn’t have a clear structure, but it does have a few important features that make it what it is. Example of Peripheral Proteins Alternative Oxidase Cytochrome c Type-II NADH Dehydrogenase Dihydroorotate Dehydrogenase Functions of Peripheral … Read more
What is Channel Protein? Types of Channel Protein There are present 2 types of channel protein such as; 1. Non-gated Channel 2. Gated Channel Structure of Channel Protein Function of Channel Protein Channel Proteins and Carrier Proteins Example of Channel Protein Chloride channel Potassium channel Calcium channel Sodium channel References
The cytoskeleton is a dynamic network of protein filaments found in the cytoplasm of all cells, including those of bacteria and archaea. It stretches from the cell nucleus to the cell membrane in eukaryotes and is composed of the same proteins in all organisms. It consists of microfilaments, intermediate filaments, and microtubules, all of which … Read more
Microtubules Definition (What are microtubules?) Occurrence of Microtubules Microtubules Structure Chemical Composition of Microtubules Intracellular Organization of Microtubules Microtubule-Associated Proteins (MAPs) Microtubule Organizing Centres (MTOCs) Assembly and Disassembly of Microtubules Function of Microtubules Microtubules serve multiple purposes in eukaryotic cells, including the following: 1. Mechanical function 2. Morphogenesis 3. Cellular polarity and motility 4. Contraction … Read more
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