Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Kinyoun stain method explained with its principle, reagents, procedure, result interpretation, modifications, applications, and Ziehl-Neelsen comparison.
Ziehl-Neelsen Staining (ZN Staining): principle, reagents, procedure, positive and negative results, AFB smear grading, modified methods, and limitations.
Lactophenol Cotton Blue (LPCB) Staining covers the principle, composition, solution preparation, fungal wet-mount procedure, microscopic results, and limitations.
Sudan Black B staining guide covering its principle, reagents, frozen-section and bacterial smear procedures, positive results, applications and limitations.
Parasporal crystal staining explained with its principle, Coomassie Brilliant Blue procedure, required materials, microscopic results, uses and limitations.
Flagella staining in microbiology – principle, reagents, Ryu and Leifson methods, step-by-step procedures, result interpretation, and limitations.
A blood smear is a simple diagnostic preparation where a drop of peripheral blood is spread on a clean slide, and it is dried for staining. It is the process used for observing blood cells and also for detecting parasites after staining methods like Giemsa. It is an important technique because it helps in demonstrating … Read more
Hanging drop technique allows for the analysis of living microorganisms. This involves fixing the Microbial suspension into a drop of liquid over the slide of glass.
Bacterial smear preparation explained with its principle, materials, procedure from broth and agar cultures, fixation methods, and precautions.
