What is Bacterial Growth Curve? The bacterial growth curve is a graphical representation of the growth of a population of bacteria in a closed system over time. It typically consists of four phases: The shape and duration of each phase can vary depending on the bacterial species, the nutrients available, and other environmental factors. Understanding … Read more
What is TaqMan Probe? Definition of TaqMan TaqMan probes are hydrolysis probes used in quantitative PCR (qPCR) to increase specificity by utilizing the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization, resulting in a fluorescence signal that allows for precise quantification of the target DNA. Principle of TaqMan TaqMan technology … Read more
Here is the list of Top 56 Pioneers in Microbiology and their Great Contribution Antonie van Leeuwenhoek (1632–1723): The First Microbiologist Antonie van Leeuwenhoek was a Dutch scientist who is often considered the father of microbiology. He was the first to observe bacteria and other microorganisms using a microscope that he designed and built himself. … Read more
What is a Zygote? A zygote is a crucial concept in biology, representing the very first stage of a new organism’s development following the fertilization event. The term “zygote” (pronounced /ˈzaɪˌɡoʊt/) originates from the Ancient Greek word ζυγωτός (zygōtós), meaning ‘joined’ or ‘yoked,’ derived from ζυγοῦν (zygoun), which means ‘to join’ or ‘to yoke’. This … Read more
What is Microbiology? Microbiology, derived from the Greek words μῑκρός (mīkros) meaning ‘small’, βίος (bíos) meaning ‘life’, and -λογία (-logía) meaning ‘study of’, is the scientific discipline focused on the study of microorganisms. These microorganisms can be unicellular (single-celled), multicellular (comprising multiple cells), or acellular (lacking cellular structure). The field encompasses several sub-disciplines, including virology, … Read more
In many domains, including medicine, statistics, and machine learning, it is vital to comprehend the complexities of diagnostic tests and their outcomes. There are four key principles that come into play when assessing a diagnostic test’s efficacy: sensitivity, specificity, false positives, and false negatives. Confusion and incorrect interpretation of test results can result from the … Read more
What is Protein Synthesis? Protein synthesis is a fundamental biological process that occurs within cells, responsible for the formation of proteins through the assembly of amino acid chains, termed polypeptides. This process is pivotal to the central dogma of genetics and is instrumental in maintaining cellular function and structure. The process of protein synthesis can … Read more
Hybridization Probes Definition Hybridization probes are a small nucleotide sequence that is used for the detection of complementary sequences (DNA or RNA) in a nucleic acid sample. DNA Probes detects the complementary DNA with the help of a hybridization reaction. This is achieved by permitting the probes to base pair with the sample nucleic acid … Read more
What is cDNA cloning? Definition of Complementary DNA or cDNA Complementary DNA (cDNA) is a form of DNA synthesized from a messenger RNA (mRNA) template through the action of the enzyme reverse transcriptase. It represents the coding sequence of genes and is commonly used in gene cloning and expression studies. cDNA Cloning Definition cDNA cloning … Read more
In molecular biology and genetics, translation is the process in which ribosomes in the cytoplasm or endoplasmic reticulum synthesize proteins after the process of transcription of DNA to RNA in the cell’s nucleus. The entire process is called gene expression.
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