What is Arabinose Operon? Structure of L-arabinose operon The linear structure of the arabinose operon consists of four unique genes and a catabolic active site. Structural genes Function of Structural genes Inducer Gene Regulatory gene Catabolic Active Site Operator Gene Promoter Gene In Summary Regulation of Arabinose Operon Negative regulation of araBAD Positive regulation of … Read more
How L-arabinose operon different from other operons? Nevertheless, Englesberg et al. discovered that the ara operon’s function differs significantly from that of the lac operon. Thus, the following paradigm was hypothesised: The arabinose system: genes and behavior How the ara system works? DNA looping: discovery, proof and biological use – helical-twist experiment
What is Operon? Definition of Operon An operon is a functional unit of DNA in prokaryotes that contains a cluster of genes under the control of a single promoter, allowing for coordinated expression of related genes. General structure of Operon The operon is a fundamental genetic regulatory system found in prokaryotes, consisting of several key … Read more
What is Intron? Origin of Introns Types of Introns Group I introns, Group II introns, Nuclear pre-mRNA introns, and Transfer RNA itrons are the four types of introns. 1. Group I introns 2. Group II Introns 3. Spliceosomal introns/Nuclear pre-mRNA Introns 4. Transfer RNA introns Intron Structure Intron Function Splicing Steps of Splicing Splicing occurs … Read more
What is Spliceosome? Properties of Spliceosome Structure and composition of Spliceosome Components of Spliceosome In addition to the pre-mRNA, spliceosomes include many other RNAs and proteins. Small nuclear ribonucleoproteins (snRNPs, pronounced “snurps”) are a type of RNA-protein complex that includes a small nuclear RNA (snRNA) bound to a protein. Gel electrophoresis enables the snRNAs to … Read more
What is 5′ capping? The unique cap structure of eukaryotic mRNA is composed of 7-methylguanosine residues connected by a 5′-5′ triphosphate bridge. Structure of 5′ cap 5’ Capping process The starting point for capping with 7-methylguanylate is the unmodified, triphosphate-terminated 5′ end of an RNA molecule. Following the last nucleotide are three phosphate groups connected … Read more
What is Heterogeneous Nuclear RNA (hnRNA)? Properties of hnRNA Processing of hnRNA During processing, the hnRNA undergoes the following chemical modifications: 1. Packaging of hnRNA with protein 2. Polyadenylation or Poly (A) tail formation 3. RNA splicing 4. 5′ Capping Mature mRNA One central coding segment, two untranslated segments, one on either side of the … Read more
What is shRNA (Short-hairpin RNA)? The shRNA is our short hairpin RNA, which is shorter, artificially manufactured, double-stranded ribonucleic acid that can be used in gene silencing investigations. What is RNA Interference? This mechanism inhibits genes through the targeting of messenger RNA molecules. mRNA is degraded, blocking protein synthesis and transcription. This is accomplished by … Read more
What is Small nuclear RNA (snRNA)? Types of Small nuclear RNA (snRNA) The snRNAs can be divided into two classes on the basis of common sequence features and protein cofactors. 1. Sm-class RNAS 2. Lsm-class RNAs Structure of snRNA Steps of snRNA gene transcription phosphorylation of the CTD of Pol II during snRNA gene transcription. … Read more
What is Enhancer RNA? Enhancer RNAs Detection Functions of enhancer RNAs Biogenesis of Enhancer RNAs 1D eRNAs 2D eRNAs Enhancer RNAs in disease Enhancer RNA and inflammation Enhancer RNA and neurodegenerative disorders Enhancer RNA and cancer Enhancer RNA in diabetes Mechanisms of Enhancer RNAs Transcriptional Noise Transcription-dependent effects Functional activity in cis Functional activity in … Read more
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