Biology Practicals - Page 46 of 50 - Biology Notes Online

Measurement Of Microorganisms – Micrometry

By Sourav Pan (M.Sc. in Microbiology) • Published on April 16, 2024 • 5 min read

Measurement Of Microorganisms - Micrometry

A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.

Giolitti-Cantoni Broth for Staphylococcus aureus

By Sourav Pan (M.Sc. in Microbiology) • Published on April 16, 2024 • 4 min read

Giolitti-Cantoni Broth for Staphylococcus aureus

Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.

Eastern blot – Protocol, Principle, Application

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 20 min read

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

Dot Blot – Protocol, Principle, Definition.

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 9 min read

Dot Blot Protocol, Principle, Definition

Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.

Restriction Digest – Protocol, Principle, result

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 8 min read

Restriction Digest Principle, Procedure, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

Reverse Blood Grouping – Principle, Procedure, Result

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 4 min read

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

Catalase Test Principle, Procedure, Result and Application.

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 8 min read

Catalase Test Principle, Procedure, Result and Application.

The catalase test is a type of biochemical test which is used to detect the production of catalase enzymes in the organism.

Xanthoproteic test – Principle, Procedure, Result, Application

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 3 min read

Xanthoproteic test Principle, Procedure, Result, Application

Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc. The presence of proteins can be confirmed qualitatively by usign several tests, Xanthoproteic test is one of them. Due to the presence of characteristic side chains in them, certain amino acids exhibit typical color reactions that form the basis for their identification.

Modified Kirby-Bauer Disc Diffusion Method

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 5 min read

Modified Kirby-Bauer Disc Diffusion Method

Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory.

Myeloperoxidase (MPO) Stain – Principle, Procedure, Result

By Sourav Pan (M.Sc. in Microbiology) • Published on April 14, 2024 • 10 min read

Myeloperoxidase is a lysosomal enzyme that can be found in the azurophil granules of neutrophils and monocytes.