Polyacrylamide Gel Electrophoresis (PAGE) refers as a biochemical technique used for separation of proteins, nucleic acids etc. according to their charge and size. In this method, separation is done by migration of charged molecules through a gel matrix. The gel matrix is polyacrylamide gel. It act as a molecular sieve. The principle is based on … Read more
SDS-PAGE is a Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. It is a laboratory technique used for separation of proteins according to their molecular weight. It is widely used in biochemical and molecular biology laboratory for protein analysis. In this method, protein sample is treated with SDS and heat. The SDS is an anionic detergent which denature … Read more
Mass Spectrometry (MS) is a analytical method. It is used for measuring mass of atoms and molecules. It is used to know the chemical composition of sample. In this method, neutral molecules are changed into charged particles. These particles are called ions. Then these ions are separated. The separation is done on the basis of … Read more
Solid State Fermentation (SSF) is a bioprocess in which microorganisms are grown on moist solid substrate. It is done in absence or near absence of free flowing water. The solid substrate acts as physical support for microbes. It also works as nutrient source. It gives carbon, nitrogen and essential minerals for growth of microorganisms. In … Read more
Infrared (IR) Spectroscopy is an analytical technique used to study and identify chemical substances by using infrared light. In this technique, the chemical bonds present in a molecule absorb infrared radiation. The bonds are not rigid, they behave like spring and show stretching and bending vibrations. When the absorbed frequency matches with the natural vibration … Read more
Nuclear Magnetic Resonance (NMR) spectroscopy is a non-destructive analytical method used for knowing the structure and composition of molecules. It is mainly used for organic compounds, biological molecules and chemical samples. It is based on magnetic nature of some atomic nuclei. Mostly hydrogen (¹H) and carbon (¹³C) nuclei are studied in this method. When sample … Read more
Thin Layer Chromatography (TLC) is an analytical technique used for separation and identification of components present in non-volatile mixtures. It is carried out on a TLC plate, which is made up of glass, plastic or aluminium sheet coated with thin layer of adsorbent material. The adsorbent material is generally silica gel or alumina, which acts … Read more
Paper Chromatography is a simple analytical technique used to separate and identify the components present in a chemical mixture. It is based on the principle of partition, where the substances are distributed between stationary phase and mobile phase. In this method, the stationary phase is usually a cellulose filter paper containing moisture within its fibers, … Read more
Gamma-ray Spectroscopy is a non-destructive analytical technique used for qualitative and quantitative study of gamma-ray energy spectra. It is used to study gamma rays emitted from radioactive source. The unstable atomic nuclei emit gamma-ray photons at specific energy level. This occurs when the nuclei pass into more stable ground state. These gamma-ray emissions are different … Read more
X-Ray Spectroscopy is a technique used for study of interaction between X-rays and matter. It is mainly used for determination of elemental composition and chemical state of a sample. It also gives information about physical properties of materials. It is based on emission and absorption of X-rays by atoms. When a material is excited by … Read more
