Gel Electrophoresis System – Definition, Principle, Parts, Types, Procedure

Gel Electrophoresis System - Definition, Principle, Parts, Types, Procedure

Gel electrophoresis system is a laboratory setup used for separation of charged biological molecules. It is used for DNA, RNA and proteins. It contains a gel box, gel matrix, running buffer and power supply. The gel matrix may be agarose or polyacrylamide. The buffer conducts electric current and keeps the molecules moving properly. When electric

Colony Counter – Types, Principle, Parts, Uses, Examples

Colony Counter - Definition, Types, Parts, Principle

Colony counter is an instrument used in microbiology laboratory for counting microbial colonies grown on agar plate. It is used for bacteria, yeast, fungi and other microorganisms. It counts the number of Colony Forming Units (CFU) present on the plate. Each colony is considered as formed from one viable microbial cell or group of cells.

Reversed-Phase Chromatography – Principle, Protocol, Applications

Reversed-Phase Chromatography - Principle, Protocol, Applications

Reversed-Phase Chromatography (RPC) is a type of liquid chromatography used for separation and analysis of compounds. It separates molecules mainly according to their hydrophobic nature. In this method the stationary phase is non-polar. The stationary phase is usually silica gel attached with hydrophobic alkyl chains. Common phases are C18 and C8. The mobile phase is

Liquid Chromatography – Definition, Types, Principle, Protocol, Applications

Liquid Chromatography - Definition, Types, Principle, Protocol, Applications

Liquid Chromatography is a separation technique used to separate, identify and measure the components of a liquid mixture. It is used in analytical chemistry for the analysis of complex samples. In this technique, the sample is passed through a column with the help of a liquid solvent. The liquid solvent is called mobile phase. The