Epi-Fluorescence Microscopy – Principle, Parts, Steps, Uses

The Fluorescence Light Path

A game changer for the microscopic world is epi-fluorescence microscopy. Imagine being able to see cells as vibrant, dynamic creatures showing off their inner workings instead of just as static objects. This method, based on the principle of fluorescence, has opened up the hidden details of life at the cellular level and made the invisible … Read more

Telomerase – Structure, Mechanism, Regulation, Functions

Telomeres and Telomerase

What is Telomerase? Telomerase (terminal transferase) is a ribonucleoprotein enzyme that extends telomeres, the repetitive nucleotide sequences at the ends of eukaryotic chromosomes. Telomeres function as protective caps, stopping chromosome ends from being erroneously perceived as DNA damage or from bonding with neighboring chromosomes. Most eukaryotes use telomerase to maintain their telomeres, but some organisms, … Read more

Telomeres – Structure, Aging, Shortening, Functions

Telomeres and Telomerase

What Are Telomeres (telomere)? At the ends of linear eukaryotic chromosomes are specialized structures called telomeres. DNA degradation is prevented by repetitive nucleotide sequences of the telomere, which caps and so stabilizes chromosomal DNA during cell division. The termini of chromosomes not only have no translation product but instead have a highly important role in … Read more

Various model of Replication – Theta, rolling circle, and linear DNA replication

Various model of Replication - Theta, rolling circle, and linear DNA replication

Plasmids are small circular molecules of DNA that exist independently of chromosomal DNA within a cell. Because they replicate independently, these molecules are called replicons. The plasmids have an origin of replication known as the ori site from which replication begins. Plasmids encode a few of their own proteins, but when it comes to replication … Read more

Gluconeogenesis – Definition, Steps, Reactions, Substrates, Importance

Gluconeogenesis - Definition, Steps, Reactions, Substrates, Importance

What is Gluconeogenesis? Gluconeogenesis Definition Gluconeogenesis is a metabolic process that synthesizes glucose from non-carbohydrate precursors, such as lactate, glycerol, and amino acids, primarily in the liver and kidneys, to maintain blood glucose levels during fasting or low carbohydrate intake. Location of Gluconeogenesis – Where does Gluconeogenesis Occur? Gluconeogenesis primarily occurs in the liver, which … Read more

Galactose Metabolism – Steps, Importance, Leloir Pathway

Galactose Metabolism - Steps, Importance, Leloir Pathway

What is Galactose? Galactose is a monosaccharide sugar that is an aldohexose. It is a C-4 epimer of glucose having the same structure as glucose, except that the atoms around carbon no. 4 are arranged differently. This sugar is around 65% of the sweetness of sucrose and is less sweet. It is paired with glucose … Read more

Glycogenesis – Enzymes, Steps, Regulation, Importance

Glycogenesis Steps

What is Glycogenesis? Definition of Glycogenesis Glycogenesis is the biological process of synthesizing glycogen from glucose molecules, primarily in the liver and muscles, for energy storage and glucose homeostasis. Key Enzymes in Glycogenesis Glycogenesis is the biochemical process that converts glucose into glycogen for storage in the liver and muscle tissues. This process relies on … Read more

Glycogenolysis – Enzymes, Steps, Regulation, Functions

Glycogenolysis - Enzymes, Steps, Regulation, Functions

What is Glycogenolysis? Glycogenolysis Definition Glycogenolysis is the enzymatic process of breaking down glycogen, a stored polysaccharide, into glucose-1-phosphate and glucose to provide energy or maintain blood sugar levels during fasting or increased energy demand. Glycogenolysis Location Glycogenolysis occurs in the cytoplasm of cells, primarily in the liver and skeletal muscles, with minimal activity in … Read more

Fate of Pyruvate – Acetyl CoA, Lactate, Alcohol Formation

Fate of Pyruvate: Acetyl CoA, Lactate, Alcohol Formation.

What do you mean by Fate of Pyruvate? Pyruvate, the end product of glycolysis, represents a central intermediate in cellular metabolism. Glycolysis is a sequence of enzyme-catalyzed reactions in which glucose is broken down into two molecules of pyruvate, generating ATP and NADH in the process. This pathway, also known as the Embden-Meyerhof-Parnas (EMP) pathway, … Read more

Glycogen – Structure, Functions, Examples

Glycogen - Defintion, Structure, Functions, Examples

What is Glycogen? Definition of Glycogen Glycogen is a branched polysaccharide made of glucose units that serves as the primary form of glucose storage in animals, fungi, and bacteria. It is primarily stored in the liver and muscles and acts as a readily mobilized energy source when the body needs glucose. Characteristics of Glycogen Glycogen … Read more

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