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Although the procedure of synthesis of RNA is the same in prokaryotes as well as eukaryotes however, the process is much more complicated in eukaryotes. In eukaryotes it is possible to synthesize RNA in the nucleus.
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What is Restriction Fragment Length Polymorphism (RFLP)? Principle of Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a powerful technique used in molecular biology to analyze genetic variations and identify differences in DNA sequences. This technique relies on the principle that the DNA fragments generated by the digestion of DNA with specific … Read more
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What is a vector in biology? Vector Definition A vector is a DNA molecule or genetic material used to transfer specific genetic information between cells in molecular biology and genetic engineering. Characteristics Features of vectors The following outlines the key features and functions of vectors, providing insights into their design and effectiveness. Types of vectors … Read more
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What is DNA Polymerase? DNA polymerases Definition DNA polymerases are enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, playing a crucial role in DNA replication and repair across all living organisms. Properties of DNA Polymerase DNA polymerases possess several important properties that contribute to their role in DNA replication. These properties ensure … Read more
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What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS), also known as high-throughput sequencing, has revolutionized the field of genomics and molecular biology by allowing the sequencing of thousands to millions of DNA molecules simultaneously. It encompasses a range of different sequencing technologies, all aimed at producing large amounts of sequence data at a lower cost … Read more
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What is Protein Synthesis Regulation? Protein synthesis regulation refers to the various mechanisms by which cells control the production of proteins. Protein synthesis is the process by which cells generate proteins, which are essential molecules for the structure, function, and regulation of various cellular processes. The regulation of protein synthesis allows cells to adjust protein … Read more
28 min read What is DNA Ladder? Definition of DNA Ladder A DNA ladder is a solution containing DNA molecules of known lengths that are used as a reference to estimate the size of unknown DNA fragments separated through gel electrophoresis. Characteristics of DNA Ladder DNA ladders used for electrophoresis possess several important characteristics to ensure their effectiveness … Read more
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What is a Nucleotide? A nucleotide is a fundamental component of nucleic acids, such as DNA and RNA. It is composed of three main parts: a pentose sugar, a nitrogenous base, and a phosphate group. Nucleotides are often referred to as the building blocks of DNA due to their crucial role in forming the DNA … Read more
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What is Primer? Types of Primers There are two main types of primers: DNA primers and RNA primers. DNA primers are commonly used in vitro, specifically for PCR amplification and DNA sequencing. On the other hand, RNA primers are utilized in in vivo processes like DNA replication and cloning. 1. DNA Primers: 2. RNA Primers: … Read more
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Principle of Western blotting The principle of Western blotting involves the separation of proteins based on their size through gel electrophoresis, followed by the transfer of the separated proteins onto a solid support (blotting), and the detection of the target protein using specific antibodies (detection). In the first step, gel electrophoresis, proteins are separated based on … Read more