Sourav Pan is the founder and sole author of Biology Notes Online. He holds a Bachelor of Science (B.Sc.) degree and an M.Sc. in Microbiology from the University of Calcutta. He creates educational resources covering microbiology, immunology, biotechnology, genetics, cell biology, botany, zoology, and other life-science subjects.
His goal is to make complex biological concepts easier to understand through well-structured, student-friendly articles based on academic textbooks, peer-reviewed research, and authoritative scientific sources. In addition to writing new content, he regularly reviews and updates articles to improve their accuracy, clarity, and educational value.
Definition of Epidemiology Overview of Epidemiology Scope of Epidemiology/Objective of Epidemiology The objectives of epidemiology are to: Major Areas of Discussion in Epidemiology The Major Areas of Discussion in Epidemiology are; Population Disease frequency Disease Distribution Disease Determinants Disease Control Fields of Epidemiology
Measurement of Bacterial Growth Why Should We Measure Bacterial Growth? The following are reasons we measure bacterial growth: Methods used for Measurement of Bacterial Growth There are many methods available to measure bacterial growth. Each one is discussed below. Cell Number count The cell number counting is done by two following methods; A. Direct Method … Read more
Microbiology is among the most popular subjects after BiPC and is an important field of Biology that studies the study of fungi, bacteria virus, as well as other microorganisms that can’t be observed with eyesight. Microbiologists identify and study the behaviors of microbes under certain circumstances and the effect they may have on the environment. … Read more
When working with centrifuges you’ll encounter two different measurement units that are called the Relative Centrifugal Force (RCF) and the number of revolutions per Minute (RPM). It is essential to be aware of the fact that they are both different. If they are not used correctly (which isn’t as rare as you believe) and the … Read more
Sucrose gradient centrifugation Definition Technique for the characterization and preparation of subcellular particles. A sucrose gradient is used to fill a centrifuge tube of the type swinging-bucket design. The bottom is the most dense, and the top the least dense. The solution is then layered with a suspension of the particles. Centrifugation separates particles in … Read more
Centrifugation of blood The process of dividing whole blood into its components is known as blood separation. For research purposes, diagnostic and therapeutic reasons as well as research, whole blood may be separated. Scientists may employ several blood separation methods to achieve this. Blood separation machines that spin at high speeds exert a centripetal force, … Read more
Filtration is the most popular method to sterilize heat sensitive liquid and gases, without exposure to denaturing temperatures. Instead of killing microorganisms that cause contamination the process simply eliminates them. It is the process of choice to sterilize chemical solutions for antibiotics radioisotopes, toxic chemicals or vaccines, as well as carbohydrates, all of which are … Read more
Radiation is used to sterilize and decontaminate medical supplies (surgeon supplies, vaccines and drugs) as well as the food and beverage industries. There are two types of radiations available: ionizing or non-ionizing. Radiation sterilization isn’t widely used in the food industry because of concerns about radioactive contamination, toxic or carcinogenic products production, taste alteration, and … Read more
Direct Microscopic Count (DMC) Cell Counting Formula In Counting Chamber The number of bacterial cell per milliliter (ml) = Number of cells counted X dilution (if used) X 50,000* The factor of 50,000 is used in order to determine the cell count for 1 ml: 1 ml = 1000 mm3 = (50 times the chamber … Read more
Binomial nomenclature means a system that is used to provide a unique name to organisms, including plants, animals, microorganisms, and other living things. This naming system makes it easier to understand them better and also separates each species from others. Therefore a universally accepted naming system is essential. Binomial nomenclature definition Binomial nomenclature is a … Read more